Literature summary for 3.5.3.1 extracted from

Tommasi, S.; Elliot, D.J.; Da Boit, M.; Gray, S.R.; Lewis, B.C.; Mangoni, A.A.
Homoarginine and inhibition of human arginase activity kinetic characterization and biological relevance (2018), Sci. Rep., 8, 3697 .

Search for more literature for 3.5.3.1

Cloned(Commentary)

Cloned (Comment)	Organism
expression in HEK293T cells	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
agmatine	poor arginase 1 inhibitor	Homo sapiens
asymmetric dimethylarginine	poor arginase 1 inhibitor	Homo sapiens
homoarginine	at 2 mM arginine, 18% and 94% inhibition of arginase 2 is observed by homoarginine at the concentration of 1 and 10 mM. At 0.1 mM arginine, the conversion of arginine to ornithine by arginase 2 is inhibited by 47% and 88% in the presence of 1 mM and 10 mM homoarginine. No significant inhibition of arginase 2 is observed at physiological homoarginine concentrations; at Km concentration (3 mM) of the substrate arginine, 1 mM homoarginine inhibits arginase 1 activity by 14%, whereas a concentration of 10 mM results in 50% inhibition of the enzyme. At 0.1 mM arginine, arginase 1 inhibition by 1 mM and 10 mM homoarginine is 30% and 76%, respectively. No significant inhibitory effects of arginase 1 activity are observed with physiological concentrations of homoarginine, 0.001-0.01 mM	Homo sapiens
lysine	at 2 mM arginine, 42% and 67% inhibition of arginase 2 is observed by lasine at 1 mM and 10 mM. At 0.1 mM arginine, the conversion of arginine to ornithine by arginase 2 is inhibited by 44% and 88% in the presence of 1 mM and 10 mM lysine. No significant inhibition of arginase 2 is observed at physiological lysine concentrations; at concentrations of 1 and 10 mM, 39% and 78% inhibition of arginase 1 activity at Km concentration (3 mM) of the substrate arginine, and 44% and 81% inhibition at physiological arginine concentrations. No significant inhibitory effects of arginase 1 activity are observed with physiological concentrations of lysine, 0.1-1.0 mM	Homo sapiens
Mn2+	the rate of arginase 1-mediated ornithine formation from L-arginine is 20% higher in absence of Mn2+ supplementation; the rate of arginase 1-mediated ornithine formation from L-arginine is 20% higher in absence of Mn2+ supplementation	Homo sapiens
additional information	arginase inhibition is unlikely to play a significant role in the cardio-protective effects of homoarginine; arginase inhibition is unlikely to play a significant role in the cardio-protective effects of homoarginine	Homo sapiens
NG-monomethyl-L-arginine	poor arginase 1 inhibitor	Homo sapiens
proline	poor arginase 1 inhibitor	Homo sapiens
symmetric dimethylarginine	poor arginase 1 inhibitor	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.9	-	L-arginine	arginase 2, pH and temperature not specified in the publication	Homo sapiens
3.3	-	L-arginine	arginase 1, pH and temperature not specified in the publication	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
40000	-	SDS-PAGE	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-arginine + H2O	Homo sapiens	-	L-ornithine + urea	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P05089	-	-
Homo sapiens	P78540	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-arginine + H2O	-	Homo sapiens	L-ornithine + urea	-	?

Synonyms

Synonyms	Comment	Organism
ARG1	-	Homo sapiens
ARG2	-	Homo sapiens
arginase-1	-	Homo sapiens
arginase-2	-	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.5	-	lysine	arginase 2, pH and temperature not specified in the publication	Homo sapiens
1.73	-	homoarginine	arginase 2, pH and temperature not specified in the publication	Homo sapiens
1.79	-	lysine	arginase 1, pH and temperature not specified in the publication	Homo sapiens
6.1	-	homoarginine	arginase 1, pH and temperature not specified in the publication	Homo sapiens

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.88	-	arginase 2, pH and temperature not specified in the publication	Homo sapiens	lysine
2.52	-	arginase 2, pH and temperature not specified in the publication	Homo sapiens	homoarginine
3.64	-	arginase 1, pH and temperature not specified in the publication	Homo sapiens	lysine
8.14	-	arginase 1, pH and temperature not specified in the publication	Homo sapiens	homoarginine

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Release 2023.1 (January 2023)