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Literature summary for 3.5.3.1 extracted from

  • Garcia, A.R.; Oliveira, D.M.P.; Claudia F Amaral, A.; Jesus, J.B.; Renno Sodero, A.C.; Souza, A.M.T.; Supuran, C.T.; Vermelho, A.B.; Rodrigues, I.A.; Pinheiro, A.S.
    Leishmania infantum arginase biochemical characterization and inhibition by naturally occurring phenolic substances (2019), J. Enzyme Inhib. Med. Chem., 34, 1100-1109 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.3.1

Application

Application Comment Organism
medicine inhibition of Leishmania arginase leads to a decrease in parasite growth and infectivity and thus represents an attractive therapeutic strategy Leishmania infantum

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3) cells Leishmania infantum

Inhibitors

Inhibitors Comment Organism Structure
apigenin 7-O-beta-D-glucoside 0.1 mM, about 40% inhibition Leishmania infantum
caffeic acid 0.1 mM, 56.98% inhibition Leishmania infantum
catechin 0.1 mM, about 40% inhibition Leishmania infantum
chlorogenic acid 0.1 mM, about 30% inhibition Leishmania infantum
dihydroquercetin 0.1 mM, about 25% inhibition Leishmania infantum
eugenol 0.1 mM, about 20% inhibition Leishmania infantum
fungizone
-
 Leishmania infantum
isorhamnetin 0.1 mM, about 55% inhibition Leishmania infantum
quercetin 0.1 mM, about 70% inhibition Leishmania infantum
Rhamnetin 0.1 mM, about 50% inhibition Leishmania infantum
rhaponticin 0.1 mM, about 60% inhibition Leishmania infantum
rosmarinic acid 0.1 mM, 71.48% inhibition Leishmania infantum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1
-
 L-arginine pH 9.5, 37°C Leishmania infantum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-arginine + H2O Leishmania infantum
-
 L-ornithine + urea
-
 ?

Organism

Organism UniProt Comment Textmining
Leishmania infantum A0A145YEM9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Leishmania infantum

Source Tissue

Source Tissue Comment Organism Textmining
amastigote intracellular Leishmania infantum
-
promastigote
-
 Leishmania infantum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arginine + H2O
-
 Leishmania infantum L-ornithine + urea
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 60
-
 Leishmania infantum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 65 25°C: about 40% of maximal activity, 65°C: about 45% of maximal activity Leishmania infantum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
 Leishmania infantum

pH Range

pH Minimum pH Maximum Comment Organism
8.6 10 pH 8.6: about 55% of maximal activity, pH 10.0: about 60% of maximal activity Leishmania infantum

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00005
-
 pH 9.5, 37°C, activity in promastigote Leishmania infantum fungizone
0.0079
-
 pH 9.5, 37°C, activity in intracellular amastigote Leishmania infantum rosmarinic acid
0.0219
-
 pH 9.5, 37°C, activity in intracellular amastigote Leishmania infantum caffeic acid
0.0573
-
 pH 9.5, 37°C, activity in promastigote Leishmania infantum rosmarinic acid
0.0608
-
 pH 9.5, 37°C, activity in promastigote Leishmania infantum caffeic acid
0.287
-
 pH 9.5, 37°C, activity in intracellular amastigote Leishmania infantum catechin
0.395
-
 pH 9.5, 37°C, activity in promastigote Leishmania infantum catechin
0.818
-
 pH 9.5, 37°C, activity in promastigote Leishmania infantum isorhamnetin
0.832
-
 pH 9.5, 37°C, activity in promastigote Leishmania infantum Rhamnetin