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Literature summary for 3.5.3.1 extracted from

  • Zhang, J.; Zhang, X.; Wu, C.; Lu, D.; Guo, G.; Mao, X.; Zhang, Y.; Wang, D.C.; Li, D.; Zou, Q.
    Expression, purification and characterization of arginase from Helicobacter pylori in its apo form (2011), PLoS ONE, 6, e26205.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.3.1

Cloned(Commentary)

Cloned (Comment) Organism
expression of apo-enzyme in a Mn2+- and Co2+-free minimal medium Helicobacter pylori

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol
-
 Helicobacter pylori
dithiothreitol
-
 Helicobacter pylori
Nomega-hydroxy-nor-arginine
-
 Helicobacter pylori
S-(2-boronoethyl)-L-cysteine
-
 Helicobacter pylori

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ metal preference in decreasing order: Co2+, Ni2+, Mn2+. Heat-activation in presence of metal ion is essential for activation of apo-enzyme Helicobacter pylori
Mn2+ metal preference in decreasing order: Co2+, Ni2+, Mn2+. Heat-activation in presence of metal ion is essential for activation of apo-enzyme Helicobacter pylori
Ni2+ metal preference in decreasing order: Co2+, Ni2+, Mn2+. Heat-activation in presence of metal ion is essential for activation of apo-enzyme Helicobacter pylori

Organism

Organism UniProt Comment Textmining
Helicobacter pylori O25949
-
-

Subunits

Subunits Comment Organism
monomer apo-form of enzyme, gel fitlration Helicobacter pylori

Synonyms

Synonyms Comment Organism
RocF
-
 Helicobacter pylori

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4
-
 apo-form of enzyme Helicobacter pylori

pH Range

pH Minimum pH Maximum Comment Organism
3
-
 no residual activity Helicobacter pylori
9
-
 50% of maximum activity Helicobacter pylori

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.01
-
 pH 7.5, 37°C Helicobacter pylori dithiothreitol
0.03
-
 pH 7.5, 37°C Helicobacter pylori 2-mercaptoethanol
0.5
-
 pH 7.5, 37°C Helicobacter pylori Nomega-hydroxy-nor-arginine
1
-
 pH 7.5, 37°C Helicobacter pylori S-(2-boronoethyl)-L-cysteine