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Literature summary for 3.5.3.1 extracted from

  • Tsui, S.M.; Lam, W.M.; Lam, T.L.; Chong, H.C.; So, P.K.; Kwok, S.Y.; Arnold, S.; Cheng, P.N.; Wheatley, D.N.; Lo, W.H.; Leung, Y.C.
    Pegylated derivatives of recombinant human arginase (rhArg1) for sustained in vivo activity in cancer therapy: preparation, characterization and analysis of their pharmacodynamics in vivo and in vitro and action upon hepatocellular carcinoma cell (HCC) (2009), Cancer Cell Int., 9, 9.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.3.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli and expressed in Bacillus subtilis as a N-terminal 6xHistagged protein Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.9
-
 L-arginine native rhArg1 Homo sapiens
2.9
-
 L-arginine Km of pegylated rhArg1 (rhArg1-peg5,000 mw) Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35510
-
 MALDI-TOF-MS Homo sapiens
35511
-
 x * 35511, SDS-PAGE Homo sapiens
35560
-
 calculated Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HEP-3B cell HBsAg-positive HCC, HB-8064, liver cancer cell line Homo sapiens
-
Hep-G2 cell HBV-negative hepatoblastoma, HB-8065, liver cancer cell line Homo sapiens
-
hepatoma cell HCC Homo sapiens
-
liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
400
-
 at 30°C, pH 8.5 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arginine + H2O
-
 Homo sapiens L-ornithine + urea
-
 ?

Subunits

Subunits Comment Organism
? x * 35511, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
arginase
-
 Homo sapiens
rhArg1 recombinant human arginase Homo sapiens