Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Leishmania amazonensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
21.5 | - |
L-arginine | native enzyme | Leishmania amazonensis | |
23.9 | - |
L-arginine | native enzyme | Leishmania amazonensis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
glycosome | - |
Leishmania amazonensis | 20015 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | a manganese wash at room temperature is the best condition to purify active enzyme, activity in the presence of 10 mM Mn2+ is at least 15fold higher than in the absence of Mn2+ | Leishmania amazonensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine + H2O | Leishmania amazonensis | - |
L-ornithine + urea | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania amazonensis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HiTrap Ni2+-chelating Sepharose column chromatography | Leishmania amazonensis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
promastigote | - |
Leishmania amazonensis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
33 | - |
homogenate supernatant, in the presence of 10 mM Mn2+ | Leishmania amazonensis |
1140 | - |
35fold purified recombinant enzyme, in the presence of 10 mM Mn2+ | Leishmania amazonensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine + H2O | - |
Leishmania amazonensis | L-ornithine + urea | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | the trimeric state is the active form of the protein, X-ray scattering | Leishmania amazonensis |
Synonyms | Comment | Organism |
---|---|---|
L-arginine amidinohydrolase | - |
Leishmania amazonensis |