Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.3.1 extracted from

  • Di Costanzo, L.; Moulin, M.; Haertlein, M.; Meilleur, F.; Christianson, D.W.
    Expression, purification, assay, and crystal structure of perdeuterated human arginase I (2007), Arch. Biochem. Biophys., 465, 82-89.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.3.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapour diffusion method with 0.1 M Bis-Tris-HCl (pH 5.5), 10-20% (w/v) PEG-3350 Homo sapiens

General Stability

General Stability Organism
perdeuterated human arginase I exhibits catalytic activity essentially identical to that of the unlabeled enzyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
2(S)-amino-6-boronohexanoic acid
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.6
-
 L-arginine at pH 8.5, in H2O buffer Homo sapiens
7.7
-
 L-arginine at pH 8.5, in D2O buffer Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ manganese metalloenzyme, each monomer contains two manganese ions Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34740
-
 theoretical value for unlabeled arginase I Homo sapiens
37030
-
 perdeuterated protein, estimated from amino acid sequence Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-arginine + H2O Homo sapiens
-
 L-ornithine + urea
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P05089
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arginine + H2O
-
 Homo sapiens L-ornithine + urea
-
 ?

Subunits

Subunits Comment Organism
dimer x-ray crystallography Homo sapiens

Synonyms

Synonyms Comment Organism
arginase I
-
 Homo sapiens