Literature summary for 3.5.3.1 extracted from

Cama, E.; Colleluori, D.M.; Emig, F.A.; Shin, H.; Kim, S.W.; Kim, N.N.; Traish, A.M.; Ash, D.E.; Christianson, D.W.
Human arginase II: crystal structure and physiological role in male and female sexual arousal (2003), Biochemistry, 42, 8445-8451.

Application

Application	Comment	Organism
medicine	enzyme is a target for inhibitors used in therapeutic treatment of smooth muscle disorders, such as erectile dysfunction	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expression of wild-type and truncated mutant enzymes in Escherichia coli BL21(DE3)	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
7 mg/ml purified fully active, untruncated enzyme complexed with 5 mM inhibitor S-(2-boronoethyl)-L-cysteine in 50 mM bicine, pH 8.5, 0.003 ml mixed with 0.1 ml MnCl2 and 0.003 ml precipitant solution containing 0.1 M Tris-HCl, pH 8.3-8.5, 3 M ammonium sulfate, equilibration over 1 ml reservoir solution containing 0.1 M Tris-HCl, pH 8.3-8.5, 3 M ammonium sulfate, and 20% v/v glycerol, hanging drop vapour diffusion method at 4°C, X-ray diffraction structure determination and analysis at 2.7 A resolution	Homo sapiens

Crystallization (Comment)

Organism

7 mg/ml purified fully active, untruncated enzyme complexed with 5 mM inhibitor S-(2-boronoethyl)-L-cysteine in 50 mM bicine, pH 8.5, 0.003 ml mixed with 0.1 ml MnCl2 and 0.003 ml precipitant solution containing 0.1 M Tris-HCl, pH 8.3-8.5, 3 M ammonium sulfate, equilibration over 1 ml reservoir solution containing 0.1 M Tris-HCl, pH 8.3-8.5, 3 M ammonium sulfate, and 20% v/v glycerol, hanging drop vapour diffusion method at 4°C, X-ray diffraction structure determination and analysis at 2.7 A resolution

Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of the truncated mutant DELTAM1-V23/DELTAH331-I354 showing reduced acivity compared to the wild-type enzyme	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism
2(S)-amino-6-boronohexanoic acid	transition state analogue inhibitor	Homo sapiens
2(S)-amino-6-boronohexanoic acid	transition state analogue inhibitor, effect on female and male hemodynamics, overview	Oryctolagus cuniculus
S-(2-boronoethyl)-L-cysteine	transition state analogue inhibitor	Homo sapiens
S-(2-boronoethyl)-L-cysteine	transition state analogue inhibitor	Oryctolagus cuniculus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.3	-	L-arginine	wild-type enzyme, pH 9.5	Homo sapiens
2.4	-	L-arginine	truncated mutant enzyme, pH 9.5	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	manganese-metalloenzyme, binuclear cluster, binding structure	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-arginine + H2O	Oryctolagus cuniculus	-	L-ornithine + urea	-	ir
L-arginine + H2O	Homo sapiens	enzyme is involved in regulating L-arginine bioavailability to NO synthase in human penile corpus cavernosum smooth muscle, and in clitoral corpus cavernosum and vagina, enzyme plays a role in both female and male sexual arousal	L-ornithine + urea	-	ir

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	isozyme II	-
Oryctolagus cuniculus	-	white New Zealand rabbits, isozyme II	-

Purification (Commentary)

Purification (Comment)	Organism
partially from vaginal tissue	Oryctolagus cuniculus
recombinant wild-type and truncated mutant enzymes from Escherichia coli BL21(DE3)	Homo sapiens

Reaction

Reaction	Comment	Organism	Reaction ID
L-arginine + H2O = L-ornithine + urea	hydrolysis via a metall-activated hydroxide mechanism, substrate binding structure	Homo sapiens	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
corpus cavernosum clitoridis	-	Homo sapiens	-
corpus cavernosum clitoridis	-	Oryctolagus cuniculus	-
corpus cavernosum penis	smooth muscle	Homo sapiens	-
corpus cavernosum penis	smooth muscle	Oryctolagus cuniculus	-
additional information	extrahepatic	Homo sapiens	-
additional information	extrahepatic	Oryctolagus cuniculus	-
smooth muscle	of penile corpus cavernosum	Homo sapiens	-
smooth muscle	of penile corpus cavernosum	Oryctolagus cuniculus	-
vagina	-	Homo sapiens	-
vagina	-	Oryctolagus cuniculus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
L-arginine + H2O	-	Homo sapiens	L-ornithine + urea	-	ir
L-arginine + H2O	-	Oryctolagus cuniculus	L-ornithine + urea	-	ir
L-arginine + H2O	enzyme is involved in regulating L-arginine bioavailability to NO synthase in human penile corpus cavernosum smooth muscle, and in clitoral corpus cavernosum and vagina, enzyme plays a role in both female and male sexual arousal	Homo sapiens	L-ornithine + urea	-	ir

Subunits

Subunits	Comment	Organism
More	monomer-monomer-interactions are stabilized by a arginine-rich network of intersubunit salt bridges, sulfate counterions, and hydrogen bonds more extensive than that observed in isozyme I	Homo sapiens

Synonyms

Synonyms	Comment	Organism
arginase II	-	Homo sapiens
arginase II	-	Oryctolagus cuniculus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
203	-	L-arginine	truncated mutant enzyme, pH 9.5	Homo sapiens
231	-	L-arginine	wild-type enzyme, pH 9.5	Homo sapiens