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Literature summary for 3.5.2.9 extracted from

  • Kumar, A.; Bachhawat, A.K.
    OXP1/YKL215c encodes an ATP-dependent 5-oxoprolinase in Saccharomyces cerevisiae: functional characterization, domain structure and identification of actin-like ATP-binding motifs in eukaryotic 5-oxoprolinases (2010), FEMS Yeast Res., 10, 394-401.
    View publication on PubMed
Search for more literature for 3.5.2.9

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharamyces cerevisiae, N-terminally His-tagged Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
additional information subunit consists of two distinct domains HyuA and HyuB. Cells of a met15 deletion strain expressing either the HyuA or the HyuB domains alone cannot utilize 5-oxoproline sulfur analogue OTC as sole source of sulfur. Cells coexpressing both HyuA and HyuB domains are able to grow on OTC as a sole source of sulfur, similar to cells expressing full-length yeast 5-oxoprolinase gene Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
additional information enzyme requires ATP, a monovalent cation such as K+ or NH4+ and a divalent cation such as Mg2+ or Mn2+ Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
140000
-
 2 * 140000, SDS-PAGE Saccharomyces cerevisiae
280000
-
 gel filtration Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P28273
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-oxo-L-proline + H2O + ATP ATP is required Saccharomyces cerevisiae L-glutamate + ADP + phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 140000, SDS-PAGE Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
OXP1
-
 Saccharomyces cerevisiae

General Information

General Information Comment Organism
physiological function comparison of the growth of 5-oxoprolinase deletion strains with wild-type strains in a variety of growth conditions does not yield any discernible phenotypes. Cells of glutamate auxotroph aco1D strain expressing OXP1 are able to grow on 5-oxoproline, although the growth is very slow Saccharomyces cerevisiae