KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.043 | - |
5-oxo-L-proline | - |
Pseudomonas putida | |
0.15 | - |
MgATP2- | - |
Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | - |
Rattus norvegicus | |
K+ | maximal activity of component A as an ATPase requires 80-300 mM | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
2 protein compounds, component A exhibits 5-oxo-L-proline depedent ATPase activity, component A composed of 6 heterodimers, 6 * 51000 + 6 * 64000, SDS-PAGE, component B is a coupling protein without ATPase activity, 8 * 82000, octamer, SDS-PAGE | Pseudomonas putida |
64000 | - |
2 protein compounds, component A exhibits 5-oxo-L-proline depedent ATPase activity, component A composed of 6 heterodimers, 6 * 51000 + 6 * 64000, SDS-PAGE, component B is a coupling protein without ATPase activity, 8 * 82000, octamer, SDS-PAGE | Pseudomonas putida |
82000 | - |
2 protein compounds, component A exhibits 5-oxo-L-proline depedent ATPase activity, component A composed of 6 heterodimers, 6 * 51000 + 6 * 64000, SDS-PAGE, component B is a coupling protein without ATPase activity, 8 * 82000, octamer, SDS-PAGE | Pseudomonas putida |
115000 | - |
native molecular weight, component A, gel filtration | Pseudomonas putida |
650000 | - |
component B, gel filtration, native conditions | Pseudomonas putida |
750000 | - |
component A, gel filtration, native conditions | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-oxo-L-proline + H2O + ATP | Rattus norvegicus | - |
L-glutamate + ADP + phosphate | - |
? | |
5-oxo-L-proline + H2O + ATP | Pseudomonas putida | - |
L-glutamate + ADP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Rattus norvegicus | - |
rat | - |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
separate purification of components A and B | Pseudomonas putida |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
75 | - |
purified component B | Pseudomonas putida |
243 | - |
purified component A | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-oxo-L-proline + H2O + ATP | - |
Rattus norvegicus | L-glutamate + ADP + phosphate | - |
? | |
5-oxo-L-proline + H2O + ATP | - |
Pseudomonas putida | L-glutamate + ADP + phosphate | - |
? | |
L-2-oxothiazolidine-4-carboxylate + H2O + ATP | - |
Rattus norvegicus | L-cysteine + ADP + phosphate | - |
? | |
L-2-oxothiazolidine-4-carboxylate + H2O + ATP | - |
Pseudomonas putida | L-cysteine + ADP + phosphate | - |
? | |
MgATP2- + H2O | - |
Pseudomonas putida | ? | - |
? | |
additional information | no activity observed with 5-oxo-D-proline | Pseudomonas putida | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
polymer | 2 protein compounds, component A exhibits 5-oxo-L-proline depedent ATPase activity, component A composed of 6 heterodimers, 6 * 51000 + 6 * 64000, SDS-PAGE, component B is a coupling protein without ATPase activity, 8 * 82000, octamer, SDS-PAGE | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
component A-catalyzed 5-oxoproline-dependent cleavage of ATP | Pseudomonas putida |
9.5 | - |
component B | Pseudomonas putida |