Crystallization (Comment) | Organism |
---|---|
enzyme in complex with inhibitor NXL104, hanging drop vapor diffusion, mixing of 12 mg/ml protein solution in a 1:1 ratio with well solution containing 0.1 M HEPES and 2 M NH4H2PO4, pH 4.1, 10°C, 4-5 days, microseeding, X-ray diffraction structure determination and analysis at 2.3 A resolution | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
clavulanate | the enzyme-inhibitor adduct is completely hydrolyzed within 48 h, binding structure comparison, inhibition kinetics and mechanism, modeling, overview | Mycobacterium tuberculosis | |
trans-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]octan-2-carboxamide | NXL104, inhibits both class A and class C beta-lactamases, irreversible inhibition of enzyme BlaC by the inhibitor with a non-lactam structural scaffold, forming a carbamyl adduct with the enzyme, the enzyme-inhibitor adduct remains stable for at least 48 hours, three-dimensional crystal structure of the BlaC-NXL104 carbamyl adduct, overview. Inhibition kinetics and mechanism, modeling, overview | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28785 | - |
x * 28785, recombinant enzyme BlaC, mass spectrometry | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
gene blaC, chromosomally encoded | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
meropenem + H2O | low activity | Mycobacterium tuberculosis | (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-[[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl]-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 28785, recombinant enzyme BlaC, mass spectrometry | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
BlaC | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Mycobacterium tuberculosis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics and mechanism, modeling, overview | Mycobacterium tuberculosis |