Cloned (Comment) | Organism |
---|---|
recombinant expression in Escherichia coli strain BL21(DE3) | Salmonella enterica |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, hanging drop vapor diffusion method, mixing of 0.0025 ml of 3.5mg/ml protein in , with 0.001 ml of crystallization solution containing 0.1 M HEPES in 1.5 M sodium citrate, pH 7.5, equilibration against 1.0 ml crystallization solution, 20°C, X-ray diffraction structure determination and analyysis at 2.2 A resolution, modeling | Salmonella enterica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
clavulanate | strong inhibition by the mechanism-based inhibitor, Gln69, Arg220, Thr237, and probably Arg240A might be important in the stabilization of the clavulanate molecule, simulations, overview | Salmonella enterica | |
tazobactam | strong inhibition by the mechanism-based inhibitor | Salmonella enterica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica | - |
serovar Typhimurium, transferable plasmid blaPER-2 in a ceftibuten-resistant strain | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ceftazidime + H2O | - |
Salmonella enterica | (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino](carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
additional information | oxyimino-cephalosporin substrates, interaction and binding structure, modeling, overview | Salmonella enterica | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PER-2 | - |
Salmonella enterica |
General Information | Comment | Organism |
---|---|---|
evolution | PER-2 belongs to a small group of extended-spectrum beta-lactamases. Enzyme PER-2 is defined by the presence of a singular trans bond between residues 166 to 167, which generates an inverted omega loop, an expanded fold of this domain that results in a wide active site cavity that allows for efficient hydrolysis of antibiotics like the oxyimino-cephalosporins, and a series of exclusive interactions between residues not frequently involved in the stabilization of the active site in other class A beta-lactamases. PER beta-lactamases might be included within a cluster of evolutionarily related enzymes harboring the conserved residues Asp136 and Asn179 | Salmonella enterica |
additional information | secondary structures and conserved motifs of PER-2 and other class A beta-lactamases, presence of a hydrogen-bond network connecting Ser70-Gln69-water-Thr237-Arg220 that might be important for the proper activity and inhibition of the enzyme, strcture comparisson, simulation and modeling, overview | Salmonella enterica |