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Literature summary for 3.5.2.6 extracted from

  • Hu, Z.; Periyannan, G.; Bennett, B.; Crowder, M.W.
    Role of the Zn1 and Zn2 sites in Metallo-beta -lactamase L1 (2008), J. Am. Chem. Soc., 130, 14207-14216.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.2.6

Protein Variants

Protein Variants Comment Organism
H116C site-directed mutagenesis. Mutant binds 0.33 equiv of Zn2+ Stenotrophomonas maltophilia
H118C site-directed mutagenesis Stenotrophomonas maltophilia
H121C site-directed mutagenesis. Mutant binds 0.11 equiv of Zn2+ Stenotrophomonas maltophilia
H196C site-directed mutagenesis Stenotrophomonas maltophilia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0023
-
 nitrocefin lactamase ZnCo-L1, 1.0 Zn(2+), 1.0 Co(2+) Stenotrophomonas maltophilia
0.0036
-
 nitrocefin lactamase ZnFe-L1, 1.0 Zn(2+), 1.0 Fe(3+) Stenotrophomonas maltophilia
0.004
-
 nitrocefin lactamase ZnFe-L1, 1.2 Zn(2+), 0.9 Fe(3+) Stenotrophomonas maltophilia
0.004
-
 nitrocefin wild-typewith 1.90 equiv of Zn(2+) Stenotrophomonas maltophilia
0.0043
-
 nitrocefin lactamase 1Co-L1, 0.9 Co(2+), 0.10 Zn(2+) Stenotrophomonas maltophilia
0.0055
-
 nitrocefin lactamase 1Zn-L1, 1.0 Zn(2+) Stenotrophomonas maltophilia
0.0059
-
 nitrocefin lactamase ZnZn-L1, 2.0 Zn(2+) added Stenotrophomonas maltophilia
0.018
-
 nitrocefin H116C, not applicable Zn(2+) content Stenotrophomonas maltophilia
0.02
-
 nitrocefin H116C with 0.85 equiv of Zn(2+) Stenotrophomonas maltophilia
0.02
-
 nitrocefin lactamase CoCo-L1, 1.80 Co(2+) Stenotrophomonas maltophilia
0.053
-
 nitrocefin lactamase 1Fe-L1, 0.9 Co(2+), 0.20 Zn(2+) Stenotrophomonas maltophilia
0.072
-
 nitrocefin H121C with 0.98 equiv of Zn(2+) Stenotrophomonas maltophilia
0.099
-
 nitrocefin H121C, not applicable Zn(2+) content Stenotrophomonas maltophilia

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ mononuclear Co(2+)- and Fe(3+)-containing lactamase L1 are essentially inactive Stenotrophomonas maltophilia
Fe3+ mononuclear Co(2+)- and Fe(3+)-containing lactamase L1 are essentially inactive Stenotrophomonas maltophilia
additional information mononuclear metal ion containing and heterobimetallic analogues of the enzyme are generated and characterized using kinetic and spectroscopic studies. Heterobimetallic analogues (ZnCoL1, ZnFeL1, FeFeL1, and CoCoL1) analogues of L1 are generated, and stopped flow kinetic studies revealed that these enzymes rapidly hydrolyze nitrocefin and that there are large amounts of the reaction intermediate formed during the reaction Stenotrophomonas maltophilia
Zn2+ metalloenzyme Stenotrophomonas maltophilia

Organism

Organism UniProt Comment Textmining
Stenotrophomonas maltophilia P52700
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nitrocefin + H2O
-
 Stenotrophomonas maltophilia (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?

Synonyms

Synonyms Comment Organism
metallo-beta-lactamase L1
-
 Stenotrophomonas maltophilia

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6
-
 nitrocefin lactamase 1Fe-L1, 0.9 Co(2+), 0.20 Zn(2+) Stenotrophomonas maltophilia
11
-
 nitrocefin lactamase 1Co-L1, 0.9 Co(2+), 0.10 Zn(2+) Stenotrophomonas maltophilia
20
-
 nitrocefin lactamase ZnFe-L1, 1.0 Zn(2+), 1.0 Fe(3+) Stenotrophomonas maltophilia
24
-
 nitrocefin lactamase ZnFe-L1, 1.2 Zn(2+), 0.9 Fe(3+) Stenotrophomonas maltophilia
26
-
 nitrocefin lactamase ZnCo-L1, 1.0 Zn(2+), 1.0 Co(2+) Stenotrophomonas maltophilia
30
-
 nitrocefin lactamase 1Zn-L1, 1.0 Zn(2+) Stenotrophomonas maltophilia
39
-
 nitrocefin lactamase ZnZn-L1, 2.0 Zn(2+) added Stenotrophomonas maltophilia
63
-
 nitrocefin lactamase CoCo-L1, 1.80 Co(2+) Stenotrophomonas maltophilia