Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Aeromonas hydrophila |
Protein Variants | Comment | Organism |
---|---|---|
D120A | site-directed mutant of CphA, generated and characterized | Aeromonas hydrophila |
D120T | site-directed mutant of CphA, generated and characterized | Aeromonas hydrophila |
H118A | site-directed mutant of CphA, generated and characterized, H118A and H196A mutations lead to a decrease of at least 1000fold in activity against carbapenems compared with the wild-type enzyme | Aeromonas hydrophila |
H196A | site-directed mutant of CphA, generated and characterized, H118A and H196A mutations lead to a decrease of at least 1000fold in activity against carbapenems compared with the wild-type enzyme | Aeromonas hydrophila |
H263A | site-directed mutant of CphA, generated and characterized | Aeromonas hydrophila |
K224Q | site-directed mutant of CphA, generated and characterized | Aeromonas hydrophila |
K226Q | site-directed mutant of CphA, generated and characterized | Aeromonas hydrophila |
N116H/N220G | site-directed mutant of CphA, generated and characterized | Aeromonas hydrophila |
N116H/N220G/K224Q | site-directed mutant of CphA, generated and characterized | Aeromonas hydrophila |
T157A | site-directed mutant of CphA, generated and characterized | Aeromonas hydrophila |
V67A | site-directed mutant of CphA, generated and characterized | Aeromonas hydrophila |
V67D | site-directed mutant of CphA, generated and characterized | Aeromonas hydrophila |
V67I | site-directed mutant of CphA, generated and characterized | Aeromonas hydrophila |
V67LFKHV | site-directed mutant of CphA, generated and characterized | Aeromonas hydrophila |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | inactivation of CphA wild-type and the H118A, H196A, H263A and D120A mutants by EDTA is studied in 15 mM sodium cacodylate (pH 6.5) at 30°C in the presence of different concentrations of EDTA | Aeromonas hydrophila |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
nitrocefin | N116H/N220G mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.015 | - |
nitrocefin | H196A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.015 | - |
nitrocefin | H263A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.026 | - |
nitrocefin | D120A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.026 | - |
nitrocefin | V67A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.03 | - |
nitrocefin | H118A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.032 | - |
nitrocefin | N116H/N220G/K224Q mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.036 | - |
nitrocefin | V67D mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.05 | - |
nitrocefin | D120T mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.05 | - |
cefotaxime | N116H/N220G mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.05 | - |
nitrocefin | V67I mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.053 | - |
cephaloridine | N116H/N220G/K224Q mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.1 | - |
cefotaxime | above, wild-type, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.1 | - |
biapenem | D120A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.1 | - |
biapenem | N116H/N220G mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.1 | - |
biapenem | V67A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.11 | - |
nitrocefin | T157A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.135 | - |
nitrocefin | K224Q mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.14 | - |
cefotaxime | K226Q mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.145 | - |
cephaloridine | N116H/N220G mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.15 | - |
benzylpenicillin | N116H/N220G mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.17 | - |
biapenem | wild-type, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.18 | - |
Imipenem | V67I mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.185 | - |
Imipenem | N116H/N220G mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.21 | - |
Imipenem | D120T mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.21 | - |
benzylpenicillin | H118A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.22 | - |
Imipenem | V67A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.3 | - |
Imipenem | N116H/N220G/K224Q mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.31 | - |
Imipenem | V67D mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.33 | - |
Imipenem | D120A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.34 | - |
Imipenem | wild-type, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.38 | - |
Imipenem | K226Q mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.39 | - |
biapenem | N116H/N220G/K224Q mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.434 | - |
cefotaxime | N116H/N220G/K224Q mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.45 | - |
cefotaxime | T157A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.55 | - |
benzylpenicillin | T157A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.57 | - |
Imipenem | T157A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.7 | - |
cefotaxime | K224Q mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.73 | - |
biapenem | H118A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.73 | - |
benzylpenicillin | V67I mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.8 | - |
benzylpenicillin | K226Q mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.84 | - |
biapenem | K226Q mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.85 | - |
nitrocefin | K226Q mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.87 | - |
benzylpenicillin | wild-type, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.92 | - |
benzylpenicillin | N116H/N220G/K224Q mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
1 | - |
cephaloridine | K224Q mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
1 | - |
benzylpenicillin | V67A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
1.15 | - |
Imipenem | H118A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
1.3 | - |
nitrocefin | wild-type, 30°C, pH 6.5 | Aeromonas hydrophila | |
1.5 | - |
biapenem | above, H263A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
1.5 | - |
Imipenem | above, K224Q mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
1.5 | - |
biapenem | above, K224Q mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
1.5 | - |
biapenem | above, T157A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
1.5 | - |
biapenem | above, V67D mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
1.5 | - |
biapenem | above, V67I mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
1.5 | - |
Imipenem | above, V67LFKHV mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
1.5 | - |
biapenem | above, V67LFKHV mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
1.7 | - |
Imipenem | H263A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
1.7 | - |
cephaloridine | K226Q mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
2 | - |
nitrocefin | above, V67LFKHV mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
4.3 | - |
Imipenem | H196A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
4.5 | - |
cephaloridine | above, H118A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
4.5 | - |
cephaloridine | above, H196A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
5 | - |
benzylpenicillin | above, H196A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
5 | - |
benzylpenicillin | above, K224Q mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
5 | - |
benzylpenicillin | above, V67D mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
6 | - |
cephaloridine | wild-type, 30°C, pH 6.5 | Aeromonas hydrophila |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | zinc content is determined by ICP-MS. Position of the second inhibitory zinc ion has not been identified | Aeromonas hydrophila |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25120 | - |
H118A mutant, denatured, calculated mass | Aeromonas hydrophila |
25120 | - |
H196A mutant, denatured, calculated mass | Aeromonas hydrophila |
25120 | - |
H263A mutant, denatured, calculated mass | Aeromonas hydrophila |
25120 | - |
H263A mutant, denatured, measured mass | Aeromonas hydrophila |
25130 | - |
H118A mutant, denatured, measured mass | Aeromonas hydrophila |
25130 | - |
H196A mutant, denatured, measured mass | Aeromonas hydrophila |
25150 | - |
D120A mutant, denatured, calculated mass | Aeromonas hydrophila |
25150 | - |
D120A mutant, denatured, measured mass | Aeromonas hydrophila |
25160 | - |
N116H/R121H/K224Q mutant, denatured, calculated mass | Aeromonas hydrophila |
25160 | - |
N116H/R121H/K224Q mutant, denatured, measured mass | Aeromonas hydrophila |
25160 | - |
T157A mutant, denatured, calculated mass | Aeromonas hydrophila |
25160 | - |
T157A mutant, denatured, measured mass | Aeromonas hydrophila |
25160 | - |
V67A mutant, denatured, calculated mass | Aeromonas hydrophila |
25160 | - |
V67A mutant, denatured, measured mass | Aeromonas hydrophila |
25180 | - |
D120T mutant, denatured, calculated mass | Aeromonas hydrophila |
25180 | - |
D120T mutant, denatured, measured mass | Aeromonas hydrophila |
25190 | - |
H118A mutant, native, measured mass | Aeromonas hydrophila |
25190 | - |
H196A mutant, native, measured mass | Aeromonas hydrophila |
25190 | - |
K224Q mutant, denatured, calculated mass | Aeromonas hydrophila |
25190 | - |
K224Q mutant, denatured, measured mass | Aeromonas hydrophila |
25190 | - |
K226Q mutant, denatured, calculated mass | Aeromonas hydrophila |
25190 | - |
K226Q mutant, denatured, measured mass | Aeromonas hydrophila |
25190 | - |
wild-type, denatured, calculated mass | Aeromonas hydrophila |
25190 | - |
wild-type, denatured, measured mass | Aeromonas hydrophila |
25200 | - |
V67I mutant, denatured, calculated mass | Aeromonas hydrophila |
25200 | - |
V67I mutant, denatured, measured mass | Aeromonas hydrophila |
25210 | - |
D120A mutant, native, measured mass | Aeromonas hydrophila |
25220 | - |
H263A mutant, native, measured mass | Aeromonas hydrophila |
25220 | - |
N116H/R121H/K224Q mutant, native, measured mass | Aeromonas hydrophila |
25220 | - |
T157A mutant, native, measured mass | Aeromonas hydrophila |
25220 | - |
V67A mutant, native, measured mass | Aeromonas hydrophila |
25240 | - |
D120T mutant, native, measured mass | Aeromonas hydrophila |
25250 | - |
K224Q mutant, native, measured mass | Aeromonas hydrophila |
25250 | - |
K226Q mutant, native, measured mass | Aeromonas hydrophila |
25250 | - |
wild-type, native, measured mass | Aeromonas hydrophila |
25270 | - |
V67I mutant, native, measured mass | Aeromonas hydrophila |
25810 | - |
V67LFKHV mutant, denatured, measured mass | Aeromonas hydrophila |
25880 | - |
V67LFKHV mutant, native, measured mass, 1 Zn2+-ion | Aeromonas hydrophila |
25900 | - |
V67LFKHV mutant, denatured, calculated mass | Aeromonas hydrophila |
25940 | - |
V67LFKHV mutant, native, measured mass, 2 Zn2+-ions | Aeromonas hydrophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeromonas hydrophila | P26918 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Aeromonas hydrophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzylpenicillin + H2O | - |
Aeromonas hydrophila | (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
biapenem + H2O | - |
Aeromonas hydrophila | (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-[(6,7-dihydro-5H-pyrazolo[1,2-a][1,2,4]triazol-4-ium-6-yl)sulfanyl]-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylate | - |
? | |
carbapenem + H2O | - |
Aeromonas hydrophila | [(2R)-2,3-dihydro-1H-pyrrol-2-yl]acetic acid | - |
? | |
cefotaxime + H2O | - |
Aeromonas hydrophila | (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cephaloridine + H2O | - |
Aeromonas hydrophila | (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
imipenem + H2O | - |
Aeromonas hydrophila | (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
nitrocefin + H2O | - |
Aeromonas hydrophila | (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | His118 and His196 participate in the catalytic mechanism of the subclass B2 CphA metallo-beta-lactamase, for which an intact Asp120/Cys221/His263 site is essential | Aeromonas hydrophila |
Synonyms | Comment | Organism |
---|---|---|
carbapenemase | - |
Aeromonas hydrophila |
CphA | - |
Aeromonas hydrophila |
metallo-beta-lactamase | - |
Aeromonas hydrophila |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002 | - |
cefotaxime | above, wild-type, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.0002 | - |
nitrocefin | D120T mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.0006 | - |
cefotaxime | K226Q mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.001 | - |
cefotaxime | K224Q mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.0015 | - |
biapenem | D120A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.0024 | - |
nitrocefin | D120A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.003 | - |
nitrocefin | H263A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.004 | - |
benzylpenicillin | K226Q mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.004 | - |
benzylpenicillin | V67A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.004 | - |
nitrocefin | V67D mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.004 | - |
nitrocefin | V67I mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.005 | - |
biapenem | above, H263A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.005 | - |
nitrocefin | H118A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.005 | - |
nitrocefin | V67A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.006 | - |
nitrocefin | H196A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.006 | - |
nitrocefin | above, V67LFKHV mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.006 | - |
cephaloridine | below, wild-type, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.006 | - |
cephaloridine | K226Q mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.007 | - |
benzylpenicillin | H118A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.007 | - |
nitrocefin | K224Q mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.008 | - |
nitrocefin | wild-type, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.01 | - |
cephaloridine | above, H118A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.01 | - |
nitrocefin | K226Q mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.015 | - |
cefotaxime | N116H/N220G/K224Q mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.015 | - |
nitrocefin | T157A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.02 | - |
Imipenem | H263A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.023 | - |
cefotaxime | T157A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.024 | - |
Imipenem | D120A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.024 | - |
cephaloridine | K224Q mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.025 | - |
Imipenem | D120T mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.03 | - |
benzylpenicillin | wild-type, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.03 | - |
benzylpenicillin | above, K224Q mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.03 | - |
benzylpenicillin | above, V67D mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.03 | - |
cephaloridine | N116H/N220G/K224Q mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.08 | - |
benzylpenicillin | V67I mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.1 | - |
benzylpenicillin | above, H196A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.1 | - |
biapenem | H118A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.11 | - |
cephaloridine | above, H196A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.13 | - |
benzylpenicillin | N116H/N220G/K224Q mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.2 | - |
nitrocefin | N116H/N220G/K224Q mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.26 | - |
biapenem | N116H/N220G/K224Q mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.3 | - |
cefotaxime | N116H/N220G mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.7 | - |
nitrocefin | N116H/N220G mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.9 | - |
cephaloridine | N116H/N220G mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
0.9 | - |
Imipenem | N116H/N220G/K224Q mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
1 | - |
benzylpenicillin | T157A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
2.4 | - |
biapenem | above, V67LFKHV mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
2.4 | - |
Imipenem | H118A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
3.3 | - |
benzylpenicillin | N116H/N220G mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
5 | - |
biapenem | N116H/N220G mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
16 | - |
Imipenem | H196A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
16 | - |
Imipenem | N116H/N220G mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
30 | - |
Imipenem | above, V67LFKHV mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
50 | - |
biapenem | above, V67I mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
60 | - |
biapenem | above, K224Q mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
100 | - |
biapenem | above, V67D mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
100 | - |
biapenem | V67A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
130 | - |
biapenem | above, T157A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
300 | - |
biapenem | wild-type, 30°C, pH 6.5 | Aeromonas hydrophila | |
490 | - |
Imipenem | above, K224Q mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
1000 | - |
Imipenem | K226Q mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
1000 | - |
biapenem | K226Q mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
1000 | - |
Imipenem | V67A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
1200 | - |
Imipenem | wild-type, 30°C, pH 6.5 | Aeromonas hydrophila | |
1280 | - |
Imipenem | V67D mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
1680 | - |
Imipenem | T157A mutant, 30°C, pH 6.5 | Aeromonas hydrophila | |
1770 | - |
Imipenem | V67I mutant, 30°C, pH 6.5 | Aeromonas hydrophila |