Protein Variants | Comment | Organism |
---|---|---|
S287N | the AmpC variants AmpC-A and AmpC-B2 harboring the S287N substitution are obtained by mutagenesis from cephalosporinases representative of the phylogenetic groups A and B2 of Escherichia coli. Their biochemical characterization reveals that the S287N replacement leads to an important increase in the catalytic efficiency toward extended-spectrum cephalosporins in the AmpC beta-lactamase of group A only | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | for substrate cefepime, wild-type AmpC-B2 and AmpC-A, Km-value is above 1000 mM | Escherichia coli | |
0.1 | - |
cefotaxime | mutant AmpC-A-S287N | Escherichia coli | |
0.2 | - |
Imipenem | mutant AmpC-A-S287N | Escherichia coli | |
0.4 | - |
ceftazidime | mutant AmpC-A-S287N | Escherichia coli | |
0.7 | - |
cefoxitin | mutant AmpC-A-S287N | Escherichia coli | |
0.8 | - |
cefoxitin | mutant AmpC-B2-S287N | Escherichia coli | |
1 | - |
cefotaxime | mutant AmpC-B2-S287N | Escherichia coli | |
1 | - |
ceftazidime | mutant AmpC-B2-S287N | Escherichia coli | |
1 | - |
cefoxitin | wild-type AmpC-A | Escherichia coli | |
1.5 | - |
cefoxitin | wild-type AmpC-B2 | Escherichia coli | |
2 | - |
Imipenem | mutant AmpC-B2-S287N | Escherichia coli | |
5.5 | - |
cephalothin | mutant AmpC-A-S287N | Escherichia coli | |
10 | - |
Imipenem | wild-type AmpC-A | Escherichia coli | |
25 | - |
cefotaxime | wild-type AmpC-A | Escherichia coli | |
30 | - |
cephalothin | wild-type AmpC-A | Escherichia coli | |
32 | - |
Imipenem | wild-type AmpC-B2 | Escherichia coli | |
37 | - |
cefepime | mutant AmpC-B2-S287N | Escherichia coli | |
45 | - |
cephalothin | mutant AmpC-B2-S287N | Escherichia coli | |
45 | - |
cefotaxime | wild-type AmpC-B2 | Escherichia coli | |
60 | - |
cephaloridine | mutant AmpC-A-S287N | Escherichia coli | |
70 | - |
ceftazidime | wild-type AmpC-A | Escherichia coli | |
75 | - |
cefepime | mutant AmpC-A-S287N | Escherichia coli | |
85 | - |
cephalothin | wild-type AmpC-B2 | Escherichia coli | |
130 | - |
cephaloridine | mutant AmpC-B2-S287N | Escherichia coli | |
270 | - |
ceftazidime | wild-type AmpC-B2 | Escherichia coli | |
650 | - |
cephaloridine | wild-type AmpC-A | Escherichia coli | |
950 | - |
cephaloridine | wild-type AmpC-B2 | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P00811 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cefepime + H2O | - |
Escherichia coli | (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[(1-methylpyrrolidinium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
cefotaxime + H2O | - |
Escherichia coli | (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cefoxitin + H2O | - |
Escherichia coli | (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
ceftazidime + H2O | - |
Escherichia coli | (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(2-carboxypropan-2-yl)oxy]imino}acetyl]amino}(carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
cephaloridine + H2O | - |
Escherichia coli | (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
cephalothin + H2O | - |
Escherichia coli | (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
imipenem + H2O | - |
Escherichia coli | (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AmpC beta-lactamase | - |
Escherichia coli |
AmpC-A | - |
Escherichia coli |
AmpC-B2 | - |
Escherichia coli |
beta-lactamase | - |
Escherichia coli |
Cephalosporinase | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
ceftazidime | mutant AmpC-B2-S287N | Escherichia coli | |
0.045 | - |
cefotaxime | mutant AmpC-B2-S287N | Escherichia coli | |
0.06 | - |
Imipenem | mutant AmpC-A-S287N | Escherichia coli | |
0.06 | - |
Imipenem | mutant AmpC-B2-S287N | Escherichia coli | |
0.08 | - |
cefotaxime | mutant AmpC-A-S287N | Escherichia coli | |
0.08 | - |
cefoxitin | mutant AmpC-B2-S287N | Escherichia coli | |
0.09 | - |
Imipenem | wild-type AmpC-B2 | Escherichia coli | |
0.1 | - |
cefoxitin | mutant AmpC-A-S287N | Escherichia coli | |
0.1 | - |
Imipenem | wild-type AmpC-A | Escherichia coli | |
0.1 | - |
cefotaxime | wild-type AmpC-B2 | Escherichia coli | |
0.2 | - |
ceftazidime | mutant AmpC-A-S287N | Escherichia coli | |
0.2 | - |
cefotaxime | wild-type AmpC-A | Escherichia coli | |
0.25 | - |
cefepime | mutant AmpC-B2-S287N | Escherichia coli | |
0.3 | - |
cefoxitin | wild-type AmpC-A | Escherichia coli | |
0.3 | - |
ceftazidime | wild-type AmpC-B2 | Escherichia coli | |
0.4 | - |
cefoxitin | wild-type AmpC-B2 | Escherichia coli | |
0.5 | - |
ceftazidime | wild-type AmpC-A | Escherichia coli | |
1.35 | - |
cefepime | mutant AmpC-A-S287N | Escherichia coli | |
10 | - |
cephalothin | mutant AmpC-B2-S287N | Escherichia coli | |
50 | - |
cephaloridine | mutant AmpC-A-S287N | Escherichia coli | |
65 | - |
cephalothin | mutant AmpC-A-S287N | Escherichia coli | |
112 | - |
cephaloridine | mutant AmpC-B2-S287N | Escherichia coli | |
230 | - |
cephaloridine | wild-type AmpC-A | Escherichia coli | |
240 | - |
cephaloridine | wild-type AmpC-B2 | Escherichia coli | |
500 | - |
cephalothin | wild-type AmpC-A | Escherichia coli | |
540 | - |
cephalothin | wild-type AmpC-B2 | Escherichia coli |