Cloned (Comment) | Organism |
---|---|
overexpression of VIM-2 in Escherichia coli strain BL21(DE3) | Pseudomonas aeruginosa |
Crystallization (Comment) | Organism |
---|---|
purified recombinant native and oxidized VIM-2, hanging drop vapour diffusion method, mixing 0.001 ml of 5.5 mg/ml of protein in 10 mM HEPES, pH 7.5, 0.2 M NaCl, and 1 mM DTT, with 0.001 ml of precipitant solution containing 30% PEG 8000, 0.2 M Na acetate, 0.1 M Na cacodylate/cacodylic acid, pH 6.5, and 0.01 mM ZnCl2, 20°C, 3-4 days, X-ray diffraction structure determination and analysis at 1.9-2.2 A resolution | Pseudomonas aeruginosa |
X-ray data collection and structure refinement of the reduced and oxidised VIM-2 | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | zinc-containing metalloenzyme | Pseudomonas aeruginosa | |
Zn2+ | VIM-2 is a Zn-beta-lactamase | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
carbapenem + H2O | Pseudomonas aeruginosa | - |
[(2R)-2,3-dihydro-1H-pyrrol-2-yl]acetic acid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9K2N0 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a beta-lactam + H2O = a substituted beta-amino acid | VIM-2 catalytic Cys221, located in the Cys site, shows a high tendency to be strongly oxidized becoming a cysteinesulfonic residue, active site structure, overview | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carbapenem + H2O | - |
Pseudomonas aeruginosa | [(2R)-2,3-dihydro-1H-pyrrol-2-yl]acetic acid | - |
? | |
imipenem + H2O | - |
Pseudomonas aeruginosa | (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
meropenem + H2O | - |
Pseudomonas aeruginosa | (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-{[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl}-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
additional information | VIM-2 has a broad substrate hydrolysis range, which includes penicillins, cephalosporins, cephamycins, oxacephamycins and carbapenems but not monobactams. Amongst the carbapenems, VIM-2 better hydrolyses meropenem than imipenem | Pseudomonas aeruginosa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | VIM-2 secondary structure, structure comparison to other VIMs, overview | Pseudomonas aeruginosa |
More | folding of alphabeta/betaalpha sandwich, consisting of a core of beta-sheet surrounded by alpha-helices | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
carbapenem-hydrolysing MBL | - |
Pseudomonas aeruginosa |
MBL | - |
Pseudomonas aeruginosa |
metallo-beta-lactamase | - |
Pseudomonas aeruginosa |
More | VIM-2 belongs to the B1 subfamily ofMBLs | Pseudomonas aeruginosa |
Verona integron-encoded MBL | - |
Pseudomonas aeruginosa |
Verona integron-encoded MBL-2 | - |
Pseudomonas aeruginosa |
VIM-2 | - |
Pseudomonas aeruginosa |
Zn-beta-lactamase | - |
Pseudomonas aeruginosa |