Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) cells | Klebsiella pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0042 | - |
Imipenem | at 30°C in 100 mM sodium phosphate buffer, pH 7.0 | Klebsiella pneumoniae | |
0.341 | - |
cefotaxime | at 30°C in 100 mM sodium phosphate buffer, pH 7.0 | Klebsiella pneumoniae | |
0.37 | - |
benzylpenicillin | at 30°C in 100 mM sodium phosphate buffer, pH 7.0 | Klebsiella pneumoniae | |
0.394 | - |
ceftaxidime | at 30°C in 100 mM sodium phosphate buffer, pH 7.0 | Klebsiella pneumoniae | |
0.454 | - |
piperacillin | at 30°C in 100 mM sodium phosphate buffer, pH 7.0 | Klebsiella pneumoniae | |
0.506 | - |
cephaloridine | at 30°C in 100 mM sodium phosphate buffer, pH 7.0 | Klebsiella pneumoniae | |
0.577 | - |
cephalothin | at 30°C in 100 mM sodium phosphate buffer, pH 7.0 | Klebsiella pneumoniae | |
0.65 | - |
cefoxitin | at 30°C in 100 mM sodium phosphate buffer, pH 7.0 | Klebsiella pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | Q09HD0 | - |
- |
Klebsiella pneumoniae CHAK36 | Q09HD0 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzylpenicillin + H2O | - |
Klebsiella pneumoniae | (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
benzylpenicillin + H2O | - |
Klebsiella pneumoniae CHAK36 | (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
cefotaxime + H2O | - |
Klebsiella pneumoniae | (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cefotaxime + H2O | - |
Klebsiella pneumoniae CHAK36 | (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cefoxitin + H2O | - |
Klebsiella pneumoniae | (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cefoxitin + H2O | - |
Klebsiella pneumoniae CHAK36 | (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
ceftaxidime + H2O | - |
Klebsiella pneumoniae | (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(2-carboxypropan-2-yl)oxy]imino}acetyl]amino}(carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
cephaloridine + H2O | - |
Klebsiella pneumoniae | (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
cephaloridine + H2O | - |
Klebsiella pneumoniae CHAK36 | (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
cephalothin + H2O | - |
Klebsiella pneumoniae | (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cephalothin + H2O | - |
Klebsiella pneumoniae CHAK36 | (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
imipenem + H2O | - |
Klebsiella pneumoniae | (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
piperacillin + H2O | - |
Klebsiella pneumoniae | (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GES-5 | an extended-spectrum class A beta-lactamase | Klebsiella pneumoniae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
ceftaxidime | at 30°C in 100 mM sodium phosphate buffer, pH 7.0 | Klebsiella pneumoniae | |
1.2 | - |
Imipenem | at 30°C in 100 mM sodium phosphate buffer, pH 7.0 | Klebsiella pneumoniae | |
2.9 | - |
cefotaxime | at 30°C in 100 mM sodium phosphate buffer, pH 7.0 | Klebsiella pneumoniae | |
4 | - |
piperacillin | at 30°C in 100 mM sodium phosphate buffer, pH 7.0 | Klebsiella pneumoniae | |
9.6 | - |
cefoxitin | at 30°C in 100 mM sodium phosphate buffer, pH 7.0 | Klebsiella pneumoniae | |
49.7 | - |
cephalothin | at 30°C in 100 mM sodium phosphate buffer, pH 7.0 | Klebsiella pneumoniae | |
190 | - |
cephaloridine | at 30°C in 100 mM sodium phosphate buffer, pH 7.0 | Klebsiella pneumoniae | |
317 | - |
benzylpenicillin | at 30°C in 100 mM sodium phosphate buffer, pH 7.0 | Klebsiella pneumoniae |