Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Chryseobacterium indologenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-ophenanthroline | - |
Chryseobacterium indologenes | |
dipicolinic acid | - |
Chryseobacterium indologenes | |
EDTA | - |
Chryseobacterium indologenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
nitrocefin | in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 | Chryseobacterium indologenes | |
0.013 | - |
benzylpenicillin | in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 | Chryseobacterium indologenes | |
0.025 | - |
cefazolin | in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 | Chryseobacterium indologenes | |
0.049 | - |
cefotaxime | in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 | Chryseobacterium indologenes | |
0.15 | - |
ampicillin | in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 | Chryseobacterium indologenes | |
0.28 | - |
meropenem | in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 | Chryseobacterium indologenes | |
0.3 | - |
Imipenem | in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 | Chryseobacterium indologenes | |
0.32 | - |
carbenicillin | in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 | Chryseobacterium indologenes | |
0.375 | - |
piperacillin | in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 | Chryseobacterium indologenes | |
1.3 | - |
Sulbactam | in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 | Chryseobacterium indologenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24540 | - |
calculated from amino acid sequence | Chryseobacterium indologenes |
25000 | - |
SDS-PAGE | Chryseobacterium indologenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chryseobacterium indologenes | Q6JE29 | - |
- |
Purification (Comment) | Organism |
---|---|
Mono Q Sepharose column chromatography | Chryseobacterium indologenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ampicillin + H2O | - |
Chryseobacterium indologenes | (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
benzylpenicillin + H2O | - |
Chryseobacterium indologenes | (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
carbenicillin + H2O | - |
Chryseobacterium indologenes | (2R,4S)-2-{(R)-carboxy[2-carboxy(phenyl)acetamido]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
cefazolin + H2O | - |
Chryseobacterium indologenes | (2R)-2-[(R)-carboxy[(1H-tetrazol-1-ylacetyl)amino]methyl]-5-[[(5-methyl-1,3,4-thiadiazol-2-yl)sulfanyl]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cefotaxime + H2O | - |
Chryseobacterium indologenes | (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
imipenem + H2O | - |
Chryseobacterium indologenes | (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
meropenem + H2O | - |
Chryseobacterium indologenes | (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-{[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl}-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
additional information | no hydrolysis of ceftazidime, cefepime, moxalactam, tazobactam, and aztreonam | Chryseobacterium indologenes | ? | - |
? | |
nitrocefin + H2O | - |
Chryseobacterium indologenes | (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
piperacillin + H2O | - |
Chryseobacterium indologenes | (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
sulbactam + H2O | - |
Chryseobacterium indologenes | (2R,4S)-2-(carboxymethyl)-5,5-dimethyl-1,1-dioxo-1lambda~6~,3-thiazolidine-4-carboxylic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IND-5 | - |
Chryseobacterium indologenes |
MBL | - |
Chryseobacterium indologenes |
metallo-beta-lactamase | - |
Chryseobacterium indologenes |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 55 | the enzyme exhibits a half-life of 90 min at 40°C, 25 min at 45°C, and less than 1 min at 55°C | Chryseobacterium indologenes |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.46 | - |
cefazolin | in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 | Chryseobacterium indologenes | |
1.53 | - |
cefotaxime | in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 | Chryseobacterium indologenes | |
3.3 | - |
Sulbactam | in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 | Chryseobacterium indologenes | |
4.13 | - |
meropenem | in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 | Chryseobacterium indologenes | |
4.4 | - |
nitrocefin | in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 | Chryseobacterium indologenes | |
7.28 | - |
Imipenem | in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 | Chryseobacterium indologenes | |
10.3 | - |
ampicillin | in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 | Chryseobacterium indologenes | |
10.92 | - |
benzylpenicillin | in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 | Chryseobacterium indologenes | |
14.61 | - |
piperacillin | in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 | Chryseobacterium indologenes | |
16.96 | - |
carbenicillin | in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 | Chryseobacterium indologenes |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
dipicolinic acid | - |
Chryseobacterium indologenes | |
0.0073 | - |
1,10-ophenanthroline | - |
Chryseobacterium indologenes | |
0.815 | - |
EDTA | - |
Chryseobacterium indologenes |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Chryseobacterium indologenes | isoelectric focusing | - |
8.8 |