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Literature summary for 3.5.2.6 extracted from
- Genetic and biochemical characterization of FUS-1 (OXA-85), a narrow-spectrum class D beta-lactamase from Fusobacterium nucleatum subsp. polymorphum (2006), Antimicrob. Agents Chemother., 50, 2673-2679.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Fusobacterium nucleatum |
| expression in Escherichia coli | Fusobacterium nucleatum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| clavulanate | - |
Fusobacterium nucleatum |  |
| clavulanic acid | poorly inactivated by clavulanate | Fusobacterium nucleatum | |
| NaCl | poorly inactivated by NaCl | Fusobacterium nucleatum | |
| tazobactam | - |
Fusobacterium nucleatum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
benzylpenicillin | at 30°C | Fusobacterium nucleatum | |
| 0.023 | - |
CENTA | at 30°C | Fusobacterium nucleatum | |
| 0.036 | - |
Oxacillin | at 30°C | Fusobacterium nucleatum | |
| 0.047 | - |
cephalothin | at 30°C | Fusobacterium nucleatum | |
| 0.076 | - |
ampicillin | at 30°C | Fusobacterium nucleatum | |
| 10 | - |
benzylpenicillin | 30°C | Fusobacterium nucleatum | |
| 36 | - |
Oxacillin | 30°C | Fusobacterium nucleatum | |
| 47 | - |
cephalothin | 30°C | Fusobacterium nucleatum | |
| 76 | - |
ampicillin | 30°C | Fusobacterium nucleatum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28910 | - |
electrospray mass spectrometry, corresponding to the cleavage of the N-terminal extremity after residue Phe6 | Fusobacterium nucleatum |
| 29050 | - |
electrospray mass spectrometry, corresponding to the cleavage of the N-terminal extremity after residue Met5 | Fusobacterium nucleatum |
| 30000 | - |
gel filtration | Fusobacterium nucleatum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fusobacterium nucleatum | Q6XL56 | subsp. polymorphum NI61 | - |
| Fusobacterium nucleatum | Q6XL56 | Fusobacterium nucleatum polymorphum strain NI61 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Fusobacterium nucleatum |
| Q sepharose column chromatography | Fusobacterium nucleatum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.8 | - |
with 1 mM ampicillin as substrate, at 30°C | Fusobacterium nucleatum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ampicillin + H2O | - |
Fusobacterium nucleatum | (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| benzylpenicillin + H2O | - |
Fusobacterium nucleatum | (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| cefuroxime + H2O | - |
Fusobacterium nucleatum | (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
| CENTA + H2O | - |
Fusobacterium nucleatum | (2R)-5-{[(3-carboxy-4-nitrophenyl)sulfanyl]methyl}-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
| cephalothin + H2O | - |
Fusobacterium nucleatum | (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
| additional information | ticarcillin and cefuroxime are weakly recognized by the enzyme, while cefotaxime, ceftazidime, and cefepime are not hydrolyzed | Fusobacterium nucleatum | ? | - |
? | |
| oxacillin + H2O | - |
Fusobacterium nucleatum | (2R,4S)-2-{(R)-carboxy[(5-methyl-3-phenyl-1,2-oxazole-4-carbonyl)amino]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| ticarcillin + H2O | - |
Fusobacterium nucleatum | (2R,4S)-2-[(R)-carboxy[[(2R)-2-carboxy-2-(thiophen-3-yl)acetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| class D beta-lactamase | - |
Fusobacterium nucleatum |
| FUS-1 | - |
Fusobacterium nucleatum |
| OXA-85 | - |
Fusobacterium nucleatum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.42 | - |
CENTA | at 30°C | Fusobacterium nucleatum | |
| 4.1 | - |
cephalothin | 30°C | Fusobacterium nucleatum | |
| 4.1 | - |
cephalothin | at 30°C | Fusobacterium nucleatum | |
| 45 | - |
ampicillin | 30°C | Fusobacterium nucleatum | |
| 45 | - |
ampicillin | at 30°C | Fusobacterium nucleatum | |
| 780 | - |
Oxacillin | 30°C | Fusobacterium nucleatum | |
| 780 | - |
Oxacillin | at 30°C | Fusobacterium nucleatum | |
| 830 | - |
benzylpenicillin | 30°C | Fusobacterium nucleatum | |
| 830 | - |
benzylpenicillin | at 30°C | Fusobacterium nucleatum | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Fusobacterium nucleatum | isoelectric focusing | - |
5.3 |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0025 | - |
at 30°C | Fusobacterium nucleatum | tazobactam | |
| 0.5 | - |
at 30°C | Fusobacterium nucleatum | clavulanic acid | |
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