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Literature summary for 3.5.2.6 extracted from

  • Sauvage, E.; Fonze, E.; Quinting, B.; Galleni, M.; Frere, J.M.; Charlier, P.
    Crystal structure of the Mycobacterium fortuitum class A beta-lactamase: structural basis for broad substrate specificity (2006), Antimicrob. Agents Chemother., 50, 2516-2521.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.2.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Mycobacterium smegmatis Mycolicibacterium fortuitum

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method in 0.1 M Tris HCl buffer, pH 7.3, with 27% polyethylene glycol 10000 Mycolicibacterium fortuitum
hanging-drop vapor diffusion method, 2.13 A resolution, P2(1)2(1)2(1), a = 35.66 A, b = 73.42 A, c = 91.32 A, one molecule per asymmetric unit Mycolicibacterium fortuitum

Inhibitors

Inhibitors Comment Organism Structure
BRL42715
-
 Mycolicibacterium fortuitum

Organism

Organism UniProt Comment Textmining
Mycolicibacterium fortuitum Q59517
-
-
Mycolicibacterium fortuitum Q59517 chromosomally encoded enzyme
-

Purification (Commentary)

Purification (Comment) Organism
High Load Q Sepharose column chromatography, MonoP HR5/20 column chromatography, and Superdex 75 gel filtration Mycolicibacterium fortuitum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azlocillin + H2O
-
 Mycolicibacterium fortuitum (2R,4S)-2-[(R)-carboxy({(2R)-2-[(2-oxoimidazolidine-1-carbonyl)amino]-2-phenylacetyl}amino)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
benzylpenicillin + H2O
-
 Mycolicibacterium fortuitum (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
carbenicillin + H2O
-
 Mycolicibacterium fortuitum (2R,4S)-2-{(R)-carboxy[2-carboxy(phenyl)acetamido]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
cefotaxime + H2O
-
 Mycolicibacterium fortuitum (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cefotaxime + H2O low specific activity Mycolicibacterium fortuitum (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cefoxitin + H2O
-
 Mycolicibacterium fortuitum (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cefuroxime + H2O
-
 Mycolicibacterium fortuitum (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cephaloridine + H2O
-
 Mycolicibacterium fortuitum (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
cephalothin + H2O
-
 Mycolicibacterium fortuitum (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
imipenem + H2O
-
 Mycolicibacterium fortuitum (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
mezlocillin + H2O
-
 Mycolicibacterium fortuitum (2R,4S)-2-[(R)-carboxy{[(2R)-2-{[3-(methanesulfonyl)-2-oxoimidazolidine-1-carbonyl]amino}-2-phenylacetyl]amino}methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
additional information does not use ceftazidime as a substrate Mycolicibacterium fortuitum ?
-
 ?
nitrocefin + H2O
-
 Mycolicibacterium fortuitum (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
piperacillin + H2O
-
 Mycolicibacterium fortuitum (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?

Synonyms

Synonyms Comment Organism
class A beta-lactamase
-
 Mycolicibacterium fortuitum
MFO
-
 Mycolicibacterium fortuitum