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Literature summary for 3.5.2.6 extracted from

  • Lawung, R.; Prachayasittikul, V.; Bulow, L.
    Purification and characterization of a beta-lactamase from Haemophilus ducreyi in Escherichia coli (2001), Protein Expr. Purif., 23, 151-158.
    View publication on PubMed
Search for more literature for 3.5.2.6

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression in Escherichia coli [Haemophilus] ducreyi

Inhibitors

Inhibitors Comment Organism Structure
EDTA 18% inhibition at 3 mM [Haemophilus] ducreyi
additional information no inhibition by boric acid, cefotaxime, PMSF [Haemophilus] ducreyi
potassium clavulanate 96% inhibition at 0.005 mM [Haemophilus] ducreyi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 benzylpenicillin pH 7.5, 37°C [Haemophilus] ducreyi
0.05
-
 nitrocefin pH 7.5, 37°C [Haemophilus] ducreyi
0.06
-
 ampicillin pH 7.5, 37°C [Haemophilus] ducreyi
0.14
-
 cefoperazone pH 7.5, 37°C [Haemophilus] ducreyi
0.26
-
 carbencillin pH 7.5, 37°C [Haemophilus] ducreyi
0.45
-
 cephalothin pH 7.5, 37°C [Haemophilus] ducreyi
1.4
-
 cephaloridine pH 7.5, 37°C [Haemophilus] ducreyi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 x * 30000, SDS-PAGE [Haemophilus] ducreyi

Organism

Organism UniProt Comment Textmining
[Haemophilus] ducreyi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli, 31.3fold by ammonium sulfate precipitation and Zn2+-affinity chromatography [Haemophilus] ducreyi

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2475
-
 purified recombinant enzyme [Haemophilus] ducreyi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ampicillin + H2O
-
 [Haemophilus] ducreyi (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
benzylpenicillin + H2O best substrate [Haemophilus] ducreyi (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
carbenicillin + H2O
-
 [Haemophilus] ducreyi (2R,4S)-2-{(R)-carboxy[2-carboxy(phenyl)acetamido]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
cefoperazone + H2O
-
 [Haemophilus] ducreyi (2R)-2-[(R)-carboxy{[(2S)-2-[(4-ethyl-2,3-dioxopiperazine-1-carbonyl)amino]-2-(4-hydroxyphenyl)acetyl]amino}methyl]-5-{[(1-methyl-1H-tetrazol-5-yl)sulfanyl]methyl}-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cephaloridine + H2O
-
 [Haemophilus] ducreyi (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
cephalothin + H2O
-
 [Haemophilus] ducreyi (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
nitrocefin + H2O
-
 [Haemophilus] ducreyi (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE [Haemophilus] ducreyi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at [Haemophilus] ducreyi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at [Haemophilus] ducreyi

pI Value

Organism Comment pI Value Maximum pI Value
[Haemophilus] ducreyi
-
-
 4.9