Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli | Stenotrophomonas maltophilia |
Protein Variants | Comment | Organism |
---|---|---|
D120C | site-directed mutagenesis, reduced activity and metal binding compared to the wild-type enzyme | Stenotrophomonas maltophilia |
D120N | site-directed mutagenesis, reduced activity and metal binding compared to the wild-type enzyme | Stenotrophomonas maltophilia |
D120S | site-directed mutagenesis, reduced activity and metal binding compared to the wild-type enzyme | Stenotrophomonas maltophilia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0011 | - |
cefoxitin | wild-type enzyme, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.0041 | - |
nitrocefin | wild-type enzyme, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.0089 | - |
cephalothin | wild-type enzyme, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.013 | - |
cefaclor | wild-type enzyme, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.015 | - |
meropenem | wild-type enzyme, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.032 | - |
biapenem | wild-type enzyme, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.033 | - |
cephalothin | mutant D120C, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.038 | - |
penicillin G | wild-type enzyme, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.045 | - |
biapenem | mutant D120C, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.046 | - |
cephalothin | mutant D120S, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.053 | - |
cefoxitin | mutant D120S, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.055 | - |
cefoxitin | mutant D120C, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.055 | - |
ampicillin | wild-type enzyme, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.057 | - |
Imipenem | wild-type enzyme, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.06 | - |
meropenem | mutant D120C, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.065 | - |
Imipenem | mutant D120C, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.068 | - |
nitrocefin | mutant D120N, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.069 | - |
nitrocefin | mutant D120C, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.07 | - |
meropenem | mutant D120N, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.08 | - |
cefaclor | mutant D120S, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.081 | - |
biapenem | mutant D120S, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.094 | - |
Imipenem | mutant D120S, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.097 | - |
meropenem | mutant D120S, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.098 | - |
cefaclor | mutant D120C, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.098 | - |
cefoxitin | mutant D120N, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.103 | - |
nitrocefin | mutant D120S, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.128 | - |
biapenem | mutant D120N, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.15 | - |
cephalothin | mutant D120N, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.31 | - |
Imipenem | mutant D120N, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.384 | - |
penicillin G | mutant D120N, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.55 | - |
cefaclor | mutant D120N, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
1.33 | - |
ampicillin | mutant D120N, pH 7.0, 25°C | Stenotrophomonas maltophilia |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | absolutely required, dinuclear zinc-metalloenzyme, metal-binding Asp, the wild-type conatins 1.9 mol of Zn2+ per mol of subunit, D120 mutant enzymes show reduced metal content, overview | Stenotrophomonas maltophilia |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Stenotrophomonas maltophilia | P52700 | metallo-beta-lactamase L1 | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli | Stenotrophomonas maltophilia |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a beta-lactam + H2O = a substituted beta-amino acid | active site, Asp120 is important for catalysis, as well as substrate and Zn2+ binding | Stenotrophomonas maltophilia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ampicillin + H2O | - |
Stenotrophomonas maltophilia | (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
biapenem + H2O | - |
Stenotrophomonas maltophilia | (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-[(6,7-dihydro-5H-pyrazolo[1,2-a][1,2,4]triazol-4-ium-6-yl)sulfanyl]-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylate | - |
? | |
cefaclor + H2O | - |
Stenotrophomonas maltophilia | (2R)-2-[(R)-{[(2R)-2-amino-2-phenylacetyl]amino}(carboxy)methyl]-5-chloro-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cefoxitin + H2O | - |
Stenotrophomonas maltophilia | (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cephalotin + H2O | - |
Stenotrophomonas maltophilia | (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
imipenem + H2O | - |
Stenotrophomonas maltophilia | (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
meropenem + H2O | - |
Stenotrophomonas maltophilia | (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-{[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl}-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
nitrocefin + H2O | - |
Stenotrophomonas maltophilia | (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
penicillin G + H2O | - |
Stenotrophomonas maltophilia | (2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L1 | - |
Stenotrophomonas maltophilia |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Stenotrophomonas maltophilia |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
penicillin G | below, mutant D120C, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.01 | - |
ampicillin | below, mutant D120C, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.01 | - |
penicillin G | below, mutant D120S, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.01 | - |
ampicillin | below, mutant D120S, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.028 | - |
nitrocefin | mutant D120S, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.18 | - |
nitrocefin | mutant D120C, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.28 | - |
cefaclor | mutant D120S, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.29 | - |
meropenem | mutant D120C, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.3 | - |
biapenem | mutant D120C, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.3 | - |
cefaclor | mutant D120C, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.31 | - |
cephalothin | mutant D120C, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.34 | - |
cefoxitin | mutant D120S, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.36 | - |
Imipenem | mutant D120C, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.37 | - |
cephalothin | mutant D120S, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.5 | - |
biapenem | mutant D120S, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.56 | - |
cefoxitin | mutant D120C, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.63 | - |
Imipenem | mutant D120S, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
0.75 | - |
meropenem | mutant D120S, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
1.53 | - |
cefoxitin | mutant D120N, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
1.9 | - |
cefoxitin | wild-type enzyme, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
2.54 | - |
nitrocefin | mutant D120N, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
3.6 | - |
biapenem | mutant D120N, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
6.3 | - |
cefaclor | mutant D120N, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
8.1 | - |
cephalothin | mutant D120N, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
10.6 | - |
meropenem | mutant D120N, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
15 | - |
Imipenem | mutant D120N, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
41 | - |
nitrocefin | wild-type enzyme, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
42 | - |
cefaclor | wild-type enzyme, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
45 | - |
penicillin G | mutant D120N, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
77 | - |
ampicillin | mutant D120N, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
82 | - |
cephalothin | wild-type enzyme, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
134 | - |
biapenem | wild-type enzyme, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
157 | - |
meropenem | wild-type enzyme, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
370 | - |
Imipenem | wild-type enzyme, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
520 | - |
ampicillin | wild-type enzyme, pH 7.0, 25°C | Stenotrophomonas maltophilia | |
600 | - |
penicillin G | wild-type enzyme, pH 7.0, 25°C | Stenotrophomonas maltophilia |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Stenotrophomonas maltophilia |