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Literature summary for 3.5.2.6 extracted from

  • Thornewell, J.; Waley, G.
    Site-directed mutagenesis and substrate-induced inactivation of beta-lactamase I (1992), Biochem. J., 288, 1045-1051.
No PubMed abstract available
Search for more literature for 3.5.2.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Bacillus subtilis Bacillus cereus

Protein Variants

Protein Variants Comment Organism
A123C stability towards cloxacillin not enhanced Bacillus cereus
A77C stability towards cloxacillin not enhanced Bacillus cereus
DELTA1-17 mutant lacking the first 17 residues, first alpha helix, reduced activity, no hydrolysis of cloxacillin Bacillus cereus

Inhibitors

Inhibitors Comment Organism Structure
Cloxacillin hydrolysis or inhibition, depending on pH, temperature, ratio enzyme/cloaxacillin Bacillus cereus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.055
-
 nitrocefin
-
 Bacillus cereus
0.065
-
 benzylpenicillin
-
 Bacillus cereus
0.22
-
 cephalosporin C
-
 Bacillus cereus
0.27
-
 Cloxacillin pH 7, 20°C Bacillus cereus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Bacillus subtilis
-
-
extracellular
-
 Bacillus cereus
-
-

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
-
-
Bacillus cereus 569/H/9
-
-
-
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus cereus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzylpenicillin + H2O
-
 Bacillus cereus (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
benzylpenicillin + H2O
-
 Bacillus cereus 569/H/9 (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
cephalosporin C + H2O poor substrate, beta-lactamse I Bacillus cereus N6-[(R)-{(2R)-5-[(acetyloxy)methyl]-4-carboxy-3,6-dihydro-2H-1,3-thiazin-2-yl}(carboxy)methyl]-6-oxo-D-lysine
-
 ?
cephalosporin C + H2O poor substrate, beta-lactamse I Bacillus cereus 569/H/9 N6-[(R)-{(2R)-5-[(acetyloxy)methyl]-4-carboxy-3,6-dihydro-2H-1,3-thiazin-2-yl}(carboxy)methyl]-6-oxo-D-lysine
-
 ?
cloxacillin + H2O hydrolysis or inhibition, depending on pH, temperature, ratio enzyme/cloaxcillin Bacillus cereus (2R,4S)-2-[(R)-carboxy{[3-(2-chlorophenyl)-5-methyl-1,2-oxazole-4-carbonyl]amino}methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
cloxacillin + H2O hydrolysis or inhibition, depending on pH, temperature, ratio enzyme/cloaxcillin Bacillus cereus 569/H/9 (2R,4S)-2-[(R)-carboxy{[3-(2-chlorophenyl)-5-methyl-1,2-oxazole-4-carbonyl]amino}methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
nitrocefin + H2O
-
 Bacillus cereus (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
nitrocefin + H2O
-
 Bacillus cereus 569/H/9 (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.2
-
 cephalosporin C
-
 Bacillus cereus
34
-
 nitrocefin
-
 Bacillus cereus
2200
-
 benzylpenicillin
-
 Bacillus cereus