Any feedback?
Literature summary for 3.5.2.6 extracted from
- beta-Lactamases (Actinomycetes species) (1975), Methods Enzymol., 43, 687-698.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Streptomyces albus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.07 | - |
benzylpenicillin | - |
Streptomyces sp. | |
| 0.12 | - |
cephalothin | - |
Streptomyces sp. | |
| 0.12 | - |
cephalexin | - |
Streptomyces sp. | |
| 0.25 | - |
2,2-dimethyl-6beta-({[5-methyl-3-(2-chlorophenyl)isoxazol-4-yl]carbonyl}amino)penam-3alpha-carboxylic acid | - |
Streptomyces albus | |
| 0.26 | - |
carbenicillin | - |
Streptomyces sp. | |
| 0.29 | - |
Methicillin | - |
Streptomyces sp. | |
| 0.33 | - |
Oxacillin | - |
Streptomyces albus | |
| 0.42 | - |
2,2-dimethyl-6beta-({[5-methyl-3-(2-chlorophenyl)isoxazol-4-yl]carbonyl}amino)penam-3alpha-carboxylic acid | - |
Streptomyces sp. | |
| 0.54 | - |
Oxacillin | - |
Streptomyces sp. | |
| 0.57 | - |
ampicillin | - |
Streptomyces sp. | |
| 0.74 | - |
benzylpenicillin | - |
Streptomyces albus | |
| 0.77 | - |
Methicillin | - |
Streptomyces albus | |
| 0.9 | - |
ampicillin | - |
Streptomyces albus | |
| 1 | - |
carbenicillin | - |
Streptomyces albus | |
| 1.05 | - |
cephalosporin C | - |
Streptomyces sp. | |
| 1.33 | - |
cephalothin | - |
Streptomyces albus | |
| 2.86 | - |
cephaloglycin | - |
Streptomyces sp. | |
| 3.33 | - |
cephalosporin C | - |
Streptomyces albus | |
| 3.84 | - |
cephaloglycin | - |
Streptomyces albus | |
| 4.53 | - |
cephalexin | - |
Streptomyces albus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Streptomyces sp. | - |
- |
| extracellular | - |
Streptomyces albus | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces albus | - |
- |
- |
| Streptomyces sp. | - |
- |
- |
| Streptomyces sp. R39 | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 33 | - |
- |
Streptomyces albus |
| 198 | - |
- |
Streptomyces sp. |
Storage Stability
| Storage Stability | Organism |
|---|---|
| crude enzyme, 0°C, amberlite, pH 5, months, no loss of activity | Streptomyces albus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ampicillin + H2O | - |
Streptomyces sp. | (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| ampicillin + H2O | - |
Streptomyces albus | (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| ampicillin + H2O | - |
Streptomyces sp. R39 | (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| benzylpenicillin + H2O | - |
Streptomyces sp. | (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| benzylpenicillin + H2O | - |
Streptomyces albus | (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| benzylpenicillin + H2O | - |
Streptomyces sp. R39 | (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| carbenicillin + H2O | - |
Streptomyces sp. | (2R,4S)-2-{(R)-carboxy[2-carboxy(phenyl)acetamido]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| carbenicillin + H2O | - |
Streptomyces albus | (2R,4S)-2-{(R)-carboxy[2-carboxy(phenyl)acetamido]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| cephalexin + H2O | - |
Streptomyces sp. | (2R)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5-methyl-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
| cephalexin + H2O | - |
Streptomyces albus | (2R)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5-methyl-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
| cephalexin + H2O | - |
Streptomyces sp. R39 | (2R)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5-methyl-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
| cephaloglycin + H2O | - |
Streptomyces sp. | (2R)-5-[(acetyloxy)methyl]-2-[(R)-{[(2R)-2-amino-2-phenylacetyl]amino}(carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
| cephaloglycin + H2O | - |
Streptomyces albus | (2R)-5-[(acetyloxy)methyl]-2-[(R)-{[(2R)-2-amino-2-phenylacetyl]amino}(carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
| cephaloglycin + H2O | - |
Streptomyces sp. R39 | (2R)-5-[(acetyloxy)methyl]-2-[(R)-{[(2R)-2-amino-2-phenylacetyl]amino}(carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
| cephalosporin C + H2O | - |
Streptomyces sp. | N6-[(R)-{(2R)-5-[(acetyloxy)methyl]-4-carboxy-3,6-dihydro-2H-1,3-thiazin-2-yl}(carboxy)methyl]-6-oxo-D-lysine | - |
? | |
| cephalosporin C + H2O | - |
Streptomyces albus | N6-[(R)-{(2R)-5-[(acetyloxy)methyl]-4-carboxy-3,6-dihydro-2H-1,3-thiazin-2-yl}(carboxy)methyl]-6-oxo-D-lysine | - |
? | |
| cephalothin + H2O | - |
Streptomyces sp. | (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
| cephalothin + H2O | - |
Streptomyces albus | (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
| cephalothin + H2O | - |
Streptomyces sp. R39 | (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
| cloxacillin + H2O | - |
Streptomyces sp. | (2R,4S)-2-[(R)-carboxy{[3-(2-chlorophenyl)-5-methyl-1,2-oxazole-4-carbonyl]amino}methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| cloxacillin + H2O | - |
Streptomyces albus | (2R,4S)-2-[(R)-carboxy{[3-(2-chlorophenyl)-5-methyl-1,2-oxazole-4-carbonyl]amino}methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| methicillin + H2O | - |
Streptomyces sp. | (2R,4S)-2-[(R)-carboxy(2,6-dimethoxybenzamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| methicillin + H2O | - |
Streptomyces albus | (2R,4S)-2-[(R)-carboxy(2,6-dimethoxybenzamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| oxacillin + H2O | - |
Streptomyces sp. | (2R,4S)-2-{(R)-carboxy[(5-methyl-3-phenyl-1,2-oxazole-4-carbonyl)amino]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| oxacillin + H2O | - |
Streptomyces albus | (2R,4S)-2-{(R)-carboxy[(5-methyl-3-phenyl-1,2-oxazole-4-carbonyl)amino]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Streptomyces sp. |
| 60 | - |
total loss of activity | Streptomyces albus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | - |
Streptomyces albus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
