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Literature summary for 3.5.2.5 extracted from
- Allantoinase and dihydroorotase binding and inhibition by flavonols and the substrates of cyclic amidohydrolases (2014), Biochimie, 101, 113-122.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Salmonella enterica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dihydroorotate | competitive inhibition | Salmonella enterica |  |
| galangin | - |
Salmonella enterica | |
| hydantoin | competitive inhibition | Salmonella enterica | |
| kaempferol | - |
Salmonella enterica | |
| additional information | binding and inhibition of allantoinase by flavonols and the substrates of other cyclic amidohydrolases, dissociation constants, docking analysis using three-dimensional structure model, PDB ID 3HM7, overview | Salmonella enterica | |
| myricetin | - |
Salmonella enterica | |
| quercetin | - |
Salmonella enterica | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Salmonella enterica | |
| 85.9 | - |
(S)-allantoin | pH 8.0, 25°C, recombinant enzyme | Salmonella enterica | |
| 86 | - |
(S)-allantoin | pH 8.0, 25°C, recombinant enzyme, in presence of phthalimide | Salmonella enterica | |
| 108.3 | - |
(S)-allantoin | pH 8.0, 25°C, recombinant enzyme, in presence of hydantoin | Salmonella enterica | |
| 129.6 | - |
(S)-allantoin | pH 8.0, 25°C, recombinant enzyme, in presence of dihydroorotate | Salmonella enterica | |
| 231.6 | - |
(S)-allantoin | pH 8.0, 25°C, recombinant enzyme, in presence of kaempferol | Salmonella enterica | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | binuclear metal center within the active site | Salmonella enterica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica | - |
serovar typhimurium LT2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis | Salmonella enterica |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| (S)-allantoin + H2O = allantoate | catalytic mechanism, overview | Salmonella enterica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-allantoin + H2O | absolute substrate specificity | Salmonella enterica | allantoate | - |
? | |
| additional information | dihydroorotate, hydantoin, and phthalimide are not hydrolyzed by allantoinase | Salmonella enterica | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Salmonella enterica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Salmonella enterica |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.035 | - |
pH 8.0, 25°C, recombinant enzyme | Salmonella enterica | kaempferol | |
| 18.6 | - |
pH 8.0, 25°C, recombinant enzyme | Salmonella enterica | dihydroorotate | |
| 230 | - |
pH 8.0, 25°C, recombinant enzyme | Salmonella enterica | hydantoin | |
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