Any feedback?
Literature summary for 3.5.2.5 extracted from
- Crystal structure of metal-dependent allantoinase from Escherichia coli (2009), J. Mol. Biol., 387, 1067-1074.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | inactive in absence of zinc | Escherichia coli |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D315N | no activity | Escherichia coli |
| N94D | no activity | Escherichia coli |
| N94V | no activity | Escherichia coli |
| R215K | no significant effect on enzyme activity | Escherichia coli |
| S288D | no significant effect on enzyme activity | Escherichia coli |
| S288V | no significant effect on enzyme activity | Escherichia coli |
| S317D | no activity | Escherichia coli |
| S317V | no activity | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 28.6 | - |
(S)-allantoin | wild-type allantoinase, supplementation with zinc acetate | Escherichia coli |  |
| 37.5 | - |
(S)-allantoin | selenomethionine-substituted wild-type allantoinase, no supplementation with zinc acetate | Escherichia coli | |
| 53.8 | - |
(S)-allantoin | mutant S288D, supplementation with zinc acetate | Escherichia coli | |
| 62.8 | - |
(S)-allantoin | mutant R215K, supplementation with zinc acetate | Escherichia coli | |
| 91.3 | - |
(S)-allantoin | mutant S288V, supplementation with zinc acetate | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | required | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-allantoin + H2O | Escherichia coli | final step of ureide pathway | allantoate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P77671 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-allantoin + H2O | - |
Escherichia coli | allantoate | - |
? | |
| (S)-allantoin + H2O | final step of ureide pathway | Escherichia coli | allantoate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | - |
Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| allantoinase | - |
Escherichia coli |
| Fe-allantoinase | - |
Escherichia coli |
| metal-dependent allantoinase | - |
Escherichia coli |
| zinc-amended allantoinase | - |
Escherichia coli |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
