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Literature summary for 3.5.2.5 extracted from

  • Gaines, P.J.; Tang, L.; Wisnewski, N.
    Insect allantoinase: cDNA cloning, purification, and characterization of the native protein from the cat flea, Ctenocephalides felis (2004), Insect Biochem. Mol. Biol., 34, 203-214.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloned from a hindgut and Malpighian tubule library, DNA and amino acid sequence determination and analysis, sequence comparisons Ctenocephalides felis

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information the enzyme has a predicted 22 amino acid signal peptide for the secretory pathway Ctenocephalides felis
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
processed recombinant and native enzyme, gel filtration Ctenocephalides felis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
allantoin + H2O Ctenocephalides felis step in the purine degradation pathway producing nitrogen waste for excretion allantoate
-
?

Organism

Organism UniProt Comment Textmining
Ctenocephalides felis
-
cat flea
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein different glycosylation stages as well as signal peptide cleavage lead to different subunits sizes of 53 kDa and 56 kDa Ctenocephalides felis
proteolytic modification the enzyme has a predicted 22 amino acid signal peptide for the secretory pathway Ctenocephalides felis

Purification (Commentary)

Purification (Comment) Organism
recombinant by gel filtration and affinity chromatography to near homogeneity Ctenocephalides felis

Source Tissue

Source Tissue Comment Organism Textmining
hindgut
-
Ctenocephalides felis
-
larva
-
Ctenocephalides felis
-
malpighian tubule
-
Ctenocephalides felis
-
additional information developmental expression analysis, no activity in pupae and eggs Ctenocephalides felis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
allantoin + H2O
-
Ctenocephalides felis allantoate
-
?
allantoin + H2O step in the purine degradation pathway producing nitrogen waste for excretion Ctenocephalides felis allantoate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 53000, processed recombinant and native enzyme, SDS-PAGE Ctenocephalides felis