Cloned (Comment) | Organism |
---|---|
cloned from a hindgut and Malpighian tubule library, DNA and amino acid sequence determination and analysis, sequence comparisons | Ctenocephalides felis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | the enzyme has a predicted 22 amino acid signal peptide for the secretory pathway | Ctenocephalides felis | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
processed recombinant and native enzyme, gel filtration | Ctenocephalides felis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
allantoin + H2O | Ctenocephalides felis | step in the purine degradation pathway producing nitrogen waste for excretion | allantoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ctenocephalides felis | - |
cat flea | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | different glycosylation stages as well as signal peptide cleavage lead to different subunits sizes of 53 kDa and 56 kDa | Ctenocephalides felis |
proteolytic modification | the enzyme has a predicted 22 amino acid signal peptide for the secretory pathway | Ctenocephalides felis |
Purification (Comment) | Organism |
---|---|
recombinant by gel filtration and affinity chromatography to near homogeneity | Ctenocephalides felis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hindgut | - |
Ctenocephalides felis | - |
larva | - |
Ctenocephalides felis | - |
malpighian tubule | - |
Ctenocephalides felis | - |
additional information | developmental expression analysis, no activity in pupae and eggs | Ctenocephalides felis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
allantoin + H2O | - |
Ctenocephalides felis | allantoate | - |
? | |
allantoin + H2O | step in the purine degradation pathway producing nitrogen waste for excretion | Ctenocephalides felis | allantoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 53000, processed recombinant and native enzyme, SDS-PAGE | Ctenocephalides felis |