Any feedback?
Literature summary for 3.5.2.3 extracted from
- Biochemical characterization of dihydroorotase of Leishmania donovani understanding pyrimidine metabolism through its inhibition (2016), Biochimie, 131, 45-53 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene DHO, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha, quantitative real-time PCR expression analysis | Leishmania donovani |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| biotin sulfone | - |
Leishmania donovani |  |
| kaempferol | kaempferol mediates perturbation of the pyrimidine pathway | Leishmania donovani | |
| additional information | inhibitor docking study, overview | Leishmania donovani |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Leishmania donovani | |
| 0.0281 | - |
N-carbamoyl-L-aspartate | pH 6.0, 25°C, recombinant enzyme | Leishmania donovani | |
| 0.6024 | - |
(S)-dihydroorotate | pH 8.0, 25°C, recombinant enzyme | Leishmania donovani | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | a zinc metalloenzyme | Leishmania donovani | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-dihydroorotate + H2O | Leishmania donovani | - |
N-carbamoyl-L-aspartate | - |
r | |
| (S)-dihydroorotate + H2O | Leishmania donovani BHU 1081 | - |
N-carbamoyl-L-aspartate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leishmania donovani | H9D0Z2 | - |
- |
| Leishmania donovani BHU 1081 | H9D0Z2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Leishmania donovani |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| promastigote | - |
Leishmania donovani | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-dihydroorotate + H2O | - |
Leishmania donovani | N-carbamoyl-L-aspartate | - |
r | |
| (S)-dihydroorotate + H2O | - |
Leishmania donovani BHU 1081 | N-carbamoyl-L-aspartate | - |
r | |
| N-carbamoyl-L-aspartate | preferred reaction direction | Leishmania donovani | (S)-dihydroorotate + H2O | - |
r | |
| N-carbamoyl-L-aspartate | preferred reaction direction | Leishmania donovani BHU 1081 | (S)-dihydroorotate + H2O | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 43900, about, sequence calculation, x* 48000, recombinant His6-tagged enzyme, SDS-PAGE | Leishmania donovani |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| amidohydrolase family protein | UniProt | Leishmania donovani |
| DHO | - |
Leishmania donovani |
| LdDHOase | - |
Leishmania donovani |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Leishmania donovani |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.1 | - |
(S)-dihydroorotate | pH 8.0, 25°C, recombinant enzyme | Leishmania donovani | |
| 2.1 | - |
N-carbamoyl-L-aspartate | pH 6.0, 25°C, recombinant enzyme | Leishmania donovani | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
(S)-dihydroorotate formation | Leishmania donovani |
| 8 | - |
(S)-dihydroorotate hydrolysis | Leishmania donovani |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0554 | - |
biotin sulfone | pH 8.0, 25°C, recombinant enzyme | Leishmania donovani | |
| 0.1514 | - |
kaempferol | pH 8.0, 25°C, recombinant enzyme | Leishmania donovani | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Leishmania donovani | sequence calculation | - |
6 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the amidohydrolase superfamily | Leishmania donovani |
| malfunction | a parallel salvage pathway of pyrimidine biosynthesis exists, since kaempferol cannot completely inhibit the pathway in vivo | Leishmania donovani |
| metabolism | de novo pyrimidine biosynthesis pathway is well developed and functional in protozoan parasite Leishmania donovani. The dihydroorotase (LdDHOase) is the third enzyme of the pathway | Leishmania donovani |
| additional information | homology modeling of LdDHOase showing the active site residues, overview | Leishmania donovani |
| physiological function | the enzyme catalyzes the reversible cyclization of N-carbamyl aspartate to dihydroorotate | Leishmania donovani |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.83 | - |
(S)-dihydroorotate | pH 8.0, 25°C, recombinant enzyme | Leishmania donovani | |
| 74.7 | - |
N-carbamoyl-L-aspartate | pH 6.0, 25°C, recombinant enzyme | Leishmania donovani | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
