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Literature summary for 3.5.2.2 extracted from
- Purification, enzymatic properties of a recombinant D-hydantoinase and its dissociation by zinc ion (2006), World J. Microbiol. Biotechnol., 22, 675-680.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strain pE-HDT/BL21 | Pseudomonas putida |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 97% residual activity at 1 mM | Pseudomonas putida |  |
| Cd2+ | 44% residual activity at 1 mM | Pseudomonas putida | |
| Cu2+ | 0.4% residual activity at 1 mM | Pseudomonas putida | |
| Zn2+ | 68% residual activity at 1 mM, the dissociation of purified enzyme into its subunits occurrs in the presence of 1 mM Zn2+ | Pseudomonas putida | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 79% increase of activity at 1 mM | Pseudomonas putida | |
| Mg2+ | 20% increase of activity at 1 mM | Pseudomonas putida | |
| Mn2+ | 74% increase of activity at 1 mM | Pseudomonas putida | |
| Ni2+ | 54% increase of activity at 1 mM | Pseudomonas putida | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
2 * 52000, SDS-PAGE | Pseudomonas putida |
| 103000 | - |
SDS-PAGE | Pseudomonas putida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | Q6TTR0 | - |
- |
| Pseudomonas putida YZ-26 | Q6TTR0 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate fractionation, phenyl-Sepharose column chromatography, and Sephacryl S-200 gel filtration | Pseudomonas putida |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10.65 | - |
after 3.4fold purification, using DL-5-phenylhydantoin as a substrate, in 50 mM Tris-HCl buffer, pH 8.0 | Pseudomonas putida |
| 11.84 | - |
after 3.4fold purification, using DL-p-hydroxyphenylhydantoin as a substrate, in 50 mM Tris-HCl buffer, pH 8.0 | Pseudomonas putida |
| 14.3 | - |
crude extract, using DL-hydantoin as a substrate, in 50 mM Tris-HCl buffer, pH 8.0 | Pseudomonas putida |
| 48.5 | - |
after 3.4fold purification, using DL-hydantoin as a substrate, in 50 mM Tris-HCl buffer, pH 8.0 | Pseudomonas putida |
| 600.8 | - |
after 3.4fold purification, using 5,6-dihydrouracil as a substrate, in 50 mM Tris-HCl buffer, pH 8.0 | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,6-dihydrouracil + H2O | - |
Pseudomonas putida | N-carbamoyl-beta-alanine | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Pseudomonas putida YZ-26 | N-carbamoyl-beta-alanine | - |
? | |
| DL-5-phenylhydantoin + H2O | - |
Pseudomonas putida | N-carbamoyl-D-phenylglycine | - |
? | |
| DL-5-phenylhydantoin + H2O | - |
Pseudomonas putida YZ-26 | N-carbamoyl-D-phenylglycine | - |
? | |
| DL-p-hydroxyphenylhydantoin + H2O | - |
Pseudomonas putida | N-carbamoyl-D-hydroxyphenylglycine | - |
? | |
| DL-p-hydroxyphenylhydantoin + H2O | - |
Pseudomonas putida YZ-26 | N-carbamoyl-D-hydroxyphenylglycine | - |
? | |
| hydantoin + H2O | - |
Pseudomonas putida | N-carbamoylglycine | - |
? | |
| hydantoin + H2O | - |
Pseudomonas putida YZ-26 | N-carbamoylglycine | - |
? | |
| additional information | no activity towards uracil and succinimide | Pseudomonas putida | ? | - |
? | |
| additional information | no activity towards uracil and succinimide | Pseudomonas putida YZ-26 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 52000, SDS-PAGE | Pseudomonas putida |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-hydantoinase | - |
Pseudomonas putida |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Pseudomonas putida |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 55 | only stable at a temperature below 50°C, activity is rapidly lost over 55°C | Pseudomonas putida |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
- |
Pseudomonas putida |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 12 | the enzymatic activity is completely lost under pH 6.0, it maintains nearly 60% activity at a pH above 12 | Pseudomonas putida |
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