Cloned (Comment) | Organism |
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- |
Pseudomonas sp. |
Crystallization (Comment) | Organism |
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Toblerone fold. Product of concatenation of three proteins of YjdF fold. PDB IDs 4BVT, 4BVS, 4BVR, 4BVQ, native enzyme and in complex with melamine, barbituric acid, and cyanuric acid and Xe-derivative, to 1.9, 2.6, 3.1, 2.6 and 2.55 A resolution, respectively. The AtzD monomer, active site and substrate all possess threefold rotational symmetry, to the extent that the active site possesses three potential SerLys catalytic dyads. A single catalytic dyad, Ser85Lys42, is hypothesized, based on biochemical evidence and crystallographic data | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
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Pseudomonas sp. | P58329 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
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additional information | hypothetical reaction mechanism consistent with a Ser85 nucleophile. Lys42 is a general base, activating Ser85 and promoting formation of a tetrahedral intermediate between Ser85 and the closest substrate carbonyl carbon, this then resolves into the acyl:enzyme intermediate following ring opening. Thereafter, a solvent water molecule is required to hydrolyse the acyl intermediate and regenerate the serine, liberating carboxybiuret | Pseudomonas sp. | ? | - |
? |