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Literature summary for 3.5.1.99 extracted from

  • Kaczocha, M.; Glaser, S.T.; Chae, J.; Brown, D.A.; Deutsch, D.G.
    Lipid droplets are novel sites of N-acylethanolamine inactivation by fatty acid amide hydrolase-2 (2010), J. Biol. Chem., 285, 2796-2806.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.99

Cloned(Commentary)

Cloned (Comment) Organism
FLAG-tagged FAAH and FAAH-2 expression in HeLa and COS-7 cells, analysis of subcellular localization of full-length and truncated enzymes in transfected cells, overview Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information FAAH-2 chimeras excluded from lipid droplets lack activity and/or are poorly expressed Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information analysis of the enzyme inhibition by quantum mechanics/molecular mechanics (QM/MM) modelling Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum FAAH Homo sapiens 5783
-
lipid droplet FAAH-2, localization of FAAH-2 on the cytoplasmic face of lipid droplets, lipid droplet localization is essential for FAAH-2 Homo sapiens 5811
-
additional information FAAH-2 is not translocated into the endoplasmic reticulum lumen Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
anandamide + H2O Homo sapiens substrate of FAAH and FAAH-2, the latter shows lower activity than FAAH arachidonic acid + ethanolamine
-
 ?
additional information Homo sapiens FAAH inactivates anandamide and other bioactive N-acylethanolamines ?
-
 ?
palmitoylethanolamide + H2O Homo sapiens substrate of FAAH and FAAH-2, the latter shows lower activity than FAAH palmitic acid + ethanolamine
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein FAAH is N-glycosylated Homo sapiens
no glycoprotein FAAH-2 does not undergo N-glycosylation Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
anandamide + H2O substrate of FAAH and FAAH-2, the latter shows lower activity than FAAH Homo sapiens arachidonic acid + ethanolamine
-
 ?
additional information FAAH inactivates anandamide and other bioactive N-acylethanolamines Homo sapiens ?
-
 ?
palmitoylethanolamide + H2O substrate of FAAH and FAAH-2, the latter shows lower activity than FAAH Homo sapiens palmitic acid + ethanolamine
-
 ?

Synonyms

Synonyms Comment Organism
FAAH
-
 Homo sapiens
FAAH-2
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 assay at Homo sapiens

General Information

General Information Comment Organism
metabolism FAAH is a key enzyme in the endocannabinoid metabolism Homo sapiens