Cloned (Comment) | Organism |
---|---|
wild-type FAAH and FAAH protein lacking the N-terminal transmembrane domain, expression in COS-7 cells and in Escherichia coli | Rattus norvegicus |
General Stability | Organism |
---|---|
100 mM Na2CO3 incubation (30 min at 4°C and pH 11.0) destroys the catalytic activity of both wild-type FAAH and FAAH protein lacking the N-terminal transmembrane domain derived from transfected COS-7 cells, whereas the native liver-isolated FAAH is stable to this treatment | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
oleamide | pH 9.0, recombinantly expressed FAAH protein lacking the N-terminal transmembrane domain | Rattus norvegicus | |
0.023 | - |
oleamide | pH 9.0, recombinantly expressed wild-type enzyme | Rattus norvegicus | |
0.031 | - |
oleamide | pH 9.0, rat liver FAAH | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | integral membrane protein | Rattus norvegicus | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinantly expressed wild-type FAAH and FAAH protein lacking the N-terminal transmembrane domain | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oleamide + H2O | - |
Rattus norvegicus | oleic acid + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | wild-type FAAH behaves as a larger oligomer than FAAH protein lacking the N-terminal transmembrane domain. Presence of SDS-resistant oligomers for wild-type FAAH, but not for FAAH protein lacking the N-terminal transmembrane domain. Self-association through the transmembrane domain is demonstrated | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
FAAH | - |
Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.3 | - |
oleamide | pH 9.0, recombinantly expressed FAAH protein lacking the N-terminal transmembrane domain | Rattus norvegicus | |
7.1 | - |
oleamide | pH 9.0, recombinantly expressed wild-type enzyme | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
native and recombinant enzymes | Rattus norvegicus |