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Literature summary for 3.5.1.98 extracted from
- Isolation and characterization of an osmotic stress and ABA induced histone deacetylase in Arachis hygogaea (2015), Front. Plant Sci., 6, 512 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene HDA1, DNA and amino acid sequence dteermination and analysis, sequence comparisons and phylogenetic analysis, functional recombinant expression of His-tagged enzyme in Escherichia coli strain BL21, quantitative RT-PCR enzyme expression analysis | Arachis hypogaea |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| trichostatinA | TSA, treatment with the specific HDAC inhibitor induces the enzyme HDA1, effectively inhibits AhHDA1 activity | Arachis hypogaea |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| nucleus | - |
Arachis hypogaea | 5634 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arachis hypogaea | A0A023IUM3 | cv. Yueyou 7 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially by isolation of nuclei from Arachis hygogaea leaves, recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography | Arachis hypogaea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower | moderate expression level | Arachis hypogaea | - |
| leaf | lowest expression level | Arachis hypogaea | - |
| mesocotyl | higher expression level | Arachis hypogaea | - |
| additional information | tissue dependent expression level of enzyme AhHDA1, overview | Arachis hypogaea | - |
| plumule | moderate expression level | Arachis hypogaea | - |
| radicle | highest expression level | Arachis hypogaea | - |
| root | high expression level | Arachis hypogaea | - |
| seedling | - |
Arachis hypogaea | - |
| stem | higher expression level | Arachis hypogaea | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 21 | - |
purified recombinant enzyme, pH 7.6, 37°C | Arachis hypogaea |
| 54.1 | - |
induced Escherichia coli cells expressing the recombinant enzyme, pH 7.6, 37°C | Arachis hypogaea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | activity assay using human histones from HeLa cells as substrate | Arachis hypogaea | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 53000, His-tagged recombinant enzyme, SDS-PAGE | Arachis hypogaea |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AhHDA1 | - |
Arachis hypogaea |
| hda1 | - |
Arachis hypogaea |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Arachis hypogaea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Arachis hypogaea |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Arachis hypogaea | the AhHDA1 encoding gene is upregulated by PEG-induced water limitation and ABA signaling | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the RPD3/HDA1-like superfamily of histone deacetylases (HDACs) | Arachis hypogaea |
| physiological function | HDA1 might be involved in the epigenetic regulation of stress resistance genes that comprise the responses to osmotic stress and abscisic acid | Arachis hypogaea |
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Release 2023.1 (January 2023)
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