Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli as glutathione S-transferase fusion protein | Thermus caldophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 1 mM, 30 min, complete loss of activity. Zn2+, Mg2+, Mn2+ may restore activity | Thermus caldophilus | |
trichostatin A | - |
Thermus caldophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km value according to commercial HDAC Fluorescent Activity Assay Kit is 0.143 mM | Thermus caldophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Zn2+, Mg2+, or Mn2+ is required. Zn2+ is the most efficient | Thermus caldophilus | |
Mn2+ | Zn2+, Mg2+, or Mn2+ is required. Zn2+ is the most efficient | Thermus caldophilus | |
Zn2+ | Zn2+, Mg2+, or Mn2+ is required. Zn2+ is the most efficient | Thermus caldophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42188 | - |
x * 42188, calculated | Thermus caldophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus caldophilus | - |
- |
- |
Thermus caldophilus GK24 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Thermus caldophilus |
Subunits | Comment | Organism |
---|---|---|
? | x * 42188, calculated | Thermus caldophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Thermus caldophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Thermus caldophilus |