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Literature summary for 3.5.1.93 extracted from
- An effective method for extraction of glutaryl-7-aminocephalosporanic acid acylase from recombinant E. coli cells (2015), Biotechnol. Bioprocess Eng., 20, 718-724 .
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression in Escherichia coli strain BL21 | Brevundimonas diminuta |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Brevundimonas diminuta |  |
| Mg2+ | - |
Brevundimonas diminuta | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cephalosporin C + H2O | Brevundimonas diminuta | - |
7-aminocephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? | |
| cephalosporin C + H2O | Brevundimonas diminuta NK703 | - |
7-aminocephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? | |
| glutaryl-7-aminocephalosporanic acid + H2O | Brevundimonas diminuta | - |
7-aminocephalosporanic acid + glutarate | - |
? | |
| glutaryl-7-aminocephalosporanic acid + H2O | Brevundimonas diminuta NK703 | - |
7-aminocephalosporanic acid + glutarate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brevundimonas diminuta | G3MDB2 | - |
- |
| Brevundimonas diminuta NK703 | G3MDB2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| several chemical treatment techniques commonly used for protein extraction are investigated for recovering glutaryl-7-aminocephalosporanic acid acylase (GLA) from recombinant Escherichia coli cells, best results are obtained by the combined use of 0.5% w/v cetyltrimethylammonium bromide (CTAB) and 0.3 M KCl at 10°C for 12 h, method evaluation and optimization of all extraction parameters, detailed overview | Brevundimonas diminuta |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.4 | - |
recombinant enzyme, crude extract, pH 8.0, 37°C | Brevundimonas diminuta |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cephalosporin C + H2O | - |
Brevundimonas diminuta | 7-aminocephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? | |
| cephalosporin C + H2O | - |
Brevundimonas diminuta NK703 | 7-aminocephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? | |
| glutaryl-7-aminocephalosporanic acid + H2O | - |
Brevundimonas diminuta | 7-aminocephalosporanic acid + glutarate | - |
? | |
| glutaryl-7-aminocephalosporanic acid + H2O | - |
Brevundimonas diminuta NK703 | 7-aminocephalosporanic acid + glutarate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GLA | - |
Brevundimonas diminuta |
| glutaryl-7-aminocephalosporanic acid acylase | - |
Brevundimonas diminuta |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Brevundimonas diminuta |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Brevundimonas diminuta |
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Release 2023.1 (January 2023)
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