Protein Variants | Comment | Organism |
---|---|---|
D445G | kcat increased, turnover rate improved up to about 10fold compared to the glutaryl-7-aminocephalosporanic acid acylase activity of gamma-glutamyltranspeptidase | Bacillus subtilis |
E423Y/E442Q/D445N | kcat/Km is increased, the catalytic efficiency improved up to 1000fold compared to the glutaryl-7-aminocephalosporanic acid acylase activity of gamma-glutamyltranspeptidase | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cephalosporin C + H2O | - |
Bacillus subtilis | 7-aminocephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glutaryl-7-aminocephalosporanic acid acylase | activity of gamma-glutamyltranspeptidase | Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0485 | - |
7-aminocephalosporanic acid | pH and temperature not specified in the publication | Bacillus subtilis | |
0.508 | - |
7-aminocephalosporanic acid | D445G mutant, pH and temperature not specified in the publication | Bacillus subtilis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.41 | - |
7-aminocephalosporanic acid | E423/E442Q/D445N mutant, pH and temperature not specified in the publication | Bacillus subtilis |