Application | Comment | Organism |
---|---|---|
pharmacology | the product of the reaction 7-aminocephalosporanic acid is a starting material for semisynthetic cephalosporin antibiotics. High thermal stability of the enzyme immobilized on silica gels indicates that it can be successfully used for the production of 7-aminocephalosporanic acid on an industrial scale | Pseudomonas sp. |
General Stability | Organism |
---|---|
the stability of the enzyme immobilized on silica gel modified with 3-aminopropyltriethoxysilane is significantly improved over its free form at lower pH values | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.9 | - |
glutaryl-7-aminocephalosporanic acid | pH 8.0, 37°C, soluble enzyme | Pseudomonas sp. | |
15.4 | - |
glutaryl-7-aminocephalosporanic acid | pH 8.0, 37°C, enzyme immobilized on silica gel modified with 3-aminopropyltriethoxysilane | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
KAC-1 | - |
Pseudomonas sp. KAC-1 | - |
KAC-1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutaryl-7-aminocephalosporanic acid + H2O | - |
Pseudomonas sp. | 7-aminocephalosporanate + glutarate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | - |
Pseudomonas sp. KAC-1 | 7-aminocephalosporanate + glutarate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GL-7-ACA acylase | - |
Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
soluble enzyme and enzyme immobilized on silica gel modified with 3-aminopropyltriethoxysilane | Pseudomonas sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | 20°C: soluble enzyme shows 35% of maximal activity, enzyme immobilized on silica gel modified with 3-aminopropyltriethoxysilane shows 45% of maximal activity, 60°C: soluble enzyme shows 20% of maximal activity, enzyme immobilized on silica gel modified with 3-aminopropyltriethoxysilane shows 40% of maximal activity | Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
thermal stability of the enzyme immobilized on silica gel modified with 3-aminopropyltriethoxysilane is increased significantly compared to that of the soluble enzyme | Pseudomonas sp. |
37 | - |
1 h, soluble enzyme and enzyme immobilized on silica gel modified with 3-aminopropyltriethoxysilane are stable, t1/2 is 307.7 min for the soluble enzyme and 733.7 min for the immobilized enzyme | Pseudomonas sp. |
45 | - |
t1/2 is 49.97 min for the soluble enzyme and 200 min for the immobilized enzyme | Pseudomonas sp. |
50 | - |
t1/2 is 29.72 min for the soluble enzyme and 65.9 min for the immobilized enzyme | Pseudomonas sp. |
55 | - |
1 h, soluble enzyme loses 77% of activity. t1/2 is 17.03 min for the soluble enzyme and 34.74 min for the immobilized enzyme | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
soluble enzyme | Pseudomonas sp. |
8.3 | - |
enzyme immobilized on silica gel modified with 3-aminopropyltriethoxysilane | Pseudomonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH-dependent activity profile for the enzyme immobilized on silica gel modified with 3-aminopropyltriethoxysilane is considerably expanded | Pseudomonas sp. |
6 | 10 | pH 6.0: soluble enzyme shows 35% of maximal activity, enzyme immobilized on silica gel modified with 3-aminopropyltriethoxysilane shows 60% of maximal activity, pH 10.0: soluble enzyme shows 67% of maximal activity, enzyme immobilized on silica gel modified with 3-aminopropyltriethoxysilane shows 75% of maximal activity | Pseudomonas sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
the stability of the enzyme immobilized on silica gel modified with 3-aminopropyltriethoxysilane is significantly improved over its free form at lower pH values | Pseudomonas sp. |