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Literature summary for 3.5.1.93 extracted from
- Deacylation activity of cephalosporin acylase to cephalosporin C is improved by changing the side-chain conformations of active-site residues (2003), Biochem. Biophys. Res. Commun., 310, 19-27.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Brevundimonas diminuta |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| F177H | beta-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 17.6% of the wild-type activity, no activity with cephalosporin C | Brevundimonas diminuta |
| F177P | beta-subunit mutant, no activity with glutaryl-7-aminocephalosporanic acid and cephalosporin C | Brevundimonas diminuta |
| F177T | beta-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 4.3% of the wild-type activity, no activity with cephalosporin C | Brevundimonas diminuta |
| L24F | beta-subunit mutant, no activity with glutaryl-7-aminocephalosporanic acid, activity with cephalosporin C is 25.9% of the wild-type activity | Brevundimonas diminuta |
| L24G | beta-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 27% of the wild-type activity, no activity with cephalosporin C | Brevundimonas diminuta |
| L24K | beta-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 1.1% of the wild-type activity, no activity with cephalosporin C | Brevundimonas diminuta |
| L24R | beta-subunit mutant, no activity with glutaryl-7-aminocephalosporanic acid, activity with cephalosporin C is 87.7% of the wild-type activity | Brevundimonas diminuta |
| L24W | beta-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 11.3% of the wild-type activity, activity with cephalosporin C is 98.8% of wild-type activity | Brevundimonas diminuta |
| Q50L | beta-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 9.9% of the wild-type activity, no activity with cephalosporin C | Brevundimonas diminuta |
| Q50M | beta-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 7.5% of the wild-type activity, activity with cephalosporin C is 180% of wild-type activity | Brevundimonas diminuta |
| Q50M/F58A | beta-subunit mutation, activity with cephalosporin C is 180% of wild-type activity | Brevundimonas diminuta |
| Q50M/F58D | beta-subunit mutation, activity with cephalosporin C is 130% of wild-type activity | Brevundimonas diminuta |
| Q50M/F58G | beta-subunit mutation, activity with cephalosporin C is 290% of wild-type activity | Brevundimonas diminuta |
| Q50M/F58H | beta-subunit mutation, activity with cephalosporin C is 160% of wild-type activity | Brevundimonas diminuta |
| Q50M/F58I | beta-subunit mutation, activity with cephalosporin C is 150% of wild-type activity | Brevundimonas diminuta |
| Q50M/F58L | beta-subunit mutation, activity with cephalosporin C is 120% of wild-type activity | Brevundimonas diminuta |
| Q50M/F58P | beta-subunit mutation, activity with cephalosporin C is 240% of wild-type activity | Brevundimonas diminuta |
| Q50M/F58S | beta-subunit mutation, activity with cephalosporin C is 260% of wild-type activity | Brevundimonas diminuta |
| Q50M/F58T | beta-subunit mutation, activity with cephalosporin C is 230% of wild-type activity | Brevundimonas diminuta |
| Q50M/Y149F | Q50M mutation in beta-subunit, Y149F mutation in alpha-subunit, activity with cephalosporin C is 157% of wild-type activity | Brevundimonas diminuta |
| Q50M/Y149K | Q50M mutation in beta-subunit, Y149K mutation in alpha-subunit, activity with cephalosporin C is 600% of wild-type activity | Brevundimonas diminuta |
| Q50M/Y149K/F177G | beta-subunit mutation, activity with cephalosporin C is 787% of wild-type activity | Brevundimonas diminuta |
| Q50M/Y149K/F177P | beta-subunit mutation, activity with cephalosporin C is 301% of wild-type activity | Brevundimonas diminuta |
| Q50M/Y149K/F177S | beta-subunit mutation, activity with cephalosporin C is 456% of wild-type activity | Brevundimonas diminuta |
| Q50M/Y149K/M145A | Q50M and Y149K are mutation in the beta-subunit, M145A is a mutation in the alpha-subunit, activity with cephalosporin C is 335% of wild-type activity | Brevundimonas diminuta |
| Q50M/Y149K/M145L | Q50M and Y149K are mutations in the beta-subunit, M145L is a mutation in the alpha-subunit, activity with cephalosporin C is 704% of wild-type activity | Brevundimonas diminuta |
| Q50M/Y149K/M145P | Q50M and Y149K are mutations in the beta-subunit, M145P is a mutation in the alpha-subunit, activity with cephalosporin C is 442% of wild-type activity | Brevundimonas diminuta |
| Q50M/Y149K/M145T | Q50M and Y149K are mutation in the beta-subunit, M145T is a mutation in the alpha-subunit, activity with cephalosporin C is 433% of wild-type activity | Brevundimonas diminuta |
| Q50M/Y149K/Y33N | beta-subunit mutation, activity with cephalosporin C is 460% of wild-type activity | Brevundimonas diminuta |
| Q50M/Y149K/Y33S | beta-subunit mutation, activity with cephalosporin C is 568% of wild-type activity | Brevundimonas diminuta |
| Q50M/Y149K/Y33T | beta-subunit mutation, activity with cephalosporin C is 424% of wild-type activity | Brevundimonas diminuta |
| Q50M/Y33D | beta-subunit mutant, activity with cephalosporin C is 450% of wild-type activity | Brevundimonas diminuta |
| Q50M/Y33G | beta-subunit mutant, activity with cephalosporin C is 70% of wild-type activity | Brevundimonas diminuta |
| Q50M/Y33H | beta-subunit mutant, activity with cephalosporin C is 101% of wild-type activity | Brevundimonas diminuta |
| Q50M/Y33L | beta-subunit mutant, activity with cephalosporin C is 202% of wild-type activity | Brevundimonas diminuta |
| Q50M/Y33N | beta-subunit mutant, activity with cephalosporin C is 350% of wild-type activity | Brevundimonas diminuta |
| Q50M/Y33P | beta-subunit mutant, activity with cephalosporin C is 99% of wild-type activity | Brevundimonas diminuta |
| Q50M/Y33S | beta-subunit mutant, activity with cephalosporin C is 60% of wild-type activity | Brevundimonas diminuta |
| Q50M/Y33T | beta-subunit mutant, activity with cephalosporin C is 109% of wild-type activity | Brevundimonas diminuta |
| Q50M/Y33V | beta-subunit mutant, activity with cephalosporin C is 135% of wild-type activity | Brevundimonas diminuta |
| Q50R | beta-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 2.1% of the wild-type activity, no activity with cephalosporin C | Brevundimonas diminuta |
| Q50T | beta-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 72.5% of the wild-type activity, no activity with cephalosporin C | Brevundimonas diminuta |
| Q50Y | beta-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 15.7% of the wild-type activity, no activity with cephalosporin C | Brevundimonas diminuta |
| R155G | alpha-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 88% of the wild-type activity | Brevundimonas diminuta |
| R57C | beta-subunit mutant, no activity with glutaryl-7-aminocephalosporanic acid and cephalosporin C | Brevundimonas diminuta |
| R57I | beta-subunit mutant, no activity with glutaryl-7-aminocephalosporanic acid and cephalosporin C | Brevundimonas diminuta |
| R57K | beta-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 0.3% of the wild-type activity, no activity with cephalosporin C | Brevundimonas diminuta |
| R57S | beta-subunit mutant, no activity with glutaryl-7-aminocephalosporanic acid and cephalosporin C | Brevundimonas diminuta |
| S152G | alpha-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 75.4% of the wild-type activity, activity with cephalosporin C is 38.6% of wild-type activity | Brevundimonas diminuta |
| S152H | alpha-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 63.7% of the wild-type activity, activity with cephalosporin C is 11.9% of wild-type activity | Brevundimonas diminuta |
| S152N | alpha-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 78% of the wild-type activity, activity with cephalosporin C is 24.8% of wild-type activity | Brevundimonas diminuta |
| S152P | alpha-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 76.2% of the wild-type activity, activity with cephalosporin C is 60.4% of wild-type activity | Brevundimonas diminuta |
| S152T | alpha-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 81.4% of the wild-type activity, activity with cephalosporin C is 34.7% of wild-type activity | Brevundimonas diminuta |
| S1A | beta-subunit mutant, no activity with glutaryl-7-aminocephalosporanic acid and cephalosporin C | Brevundimonas diminuta |
| S1betaC | no activity with glutaryl-7-aminocephalosporanic acid and cephalosporin C | Brevundimonas diminuta |
| V70G | beta-subunit mutant, no activity with glutaryl-7-aminocephalosporanic acid, activity with cephalosporin C is 32.1% of the wild-type activity | Brevundimonas diminuta |
| V70S | beta-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 28.8% of the wild-type activity, no activity with cephalosporin C | Brevundimonas diminuta |
| Y149C | alpha-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 16.4% of the wild-type activity, activity with cephalosporin C is 24% of wild-type activity | Brevundimonas diminuta |
| Y149G | alpha-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 0.7% of the wild-type activity, no activity with cephalosporin C | Brevundimonas diminuta |
| Y149L | alpha-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 6.3% of the wild-type activity, activity with cephalosporin C is 48% of wild-type activity | Brevundimonas diminuta |
| Y149N | alpha-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 30.8% of the wild-type activity, activity with cephalosporin C is 48% of wild-type activity | Brevundimonas diminuta |
| Y149P | alpha-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 1.1% of the wild-type activity, no activity with cephalosporin C | Brevundimonas diminuta |
| Y149R | alpha-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 13.6% of the wild-type activity, no activity with cephalosporin C | Brevundimonas diminuta |
| Y33F | beta-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 65.4% of the wild-type activity, no activity with cephalosporin C | Brevundimonas diminuta |
| Y33I | beta-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 9.3% of the wild-type activity, activity with cephalosporin C is 90% of wild-type activity | Brevundimonas diminuta |
| Y33S | beta-subunit mutant, no activity with glutaryl-7-aminocephalosporanic acid, activity with cephalosporin C is 120% of the wild-type activity | Brevundimonas diminuta |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brevundimonas diminuta | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Brevundimonas diminuta |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutaryl-7-aminocephalosporanic acid + H2O | - |
Brevundimonas diminuta | 7-aminocephalosporanate + glutarate | - |
? |  |
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Release 2023.1 (January 2023)
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