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Literature summary for 3.5.1.93 extracted from

  • Binder, R.; Brown, J.; Romancik, G.
    Biochemical characterization of a glutaryl-7-aminocephalosporanic acid acylase from Pseudomonas strain BL072 (1994), Appl. Environ. Microbiol., 60, 1805-1809.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.93

General Stability

General Stability Organism
Eupergit C-immobilized acylase has a half-life of greater than 16 days Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6
-
 glutaryl deacetoxy 7-aminocephalosporanic acid
-
 Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
 1 * 65000 + 1 * 24000 Pseudomonas sp.
65000
-
 1 * 65000 + 1 * 24000 Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. BL072
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cephalosporin C + H2O low activity Pseudomonas sp. cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
 ?
cephalosporin C + H2O low activity Pseudomonas sp. BL072 cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
 ?
glutaryl deacetoxy 7-aminocephalosporanic acid + H2O
-
 Pseudomonas sp. deacetoxycephalosporanic acid + glutarate
-
 ?
glutaryl deacetoxy 7-aminocephalosporanic acid + H2O
-
 Pseudomonas sp. BL072 deacetoxycephalosporanic acid + glutarate
-
 ?
glutaryl deacetyl-7-aminocephalosporanic acid + H2O
-
 Pseudomonas sp. deacetyl-7-aminocephalosporanic acid + glutarate
-
 ?
glutaryl deacetyl-7-aminocephalosporanic acid + H2O
-
 Pseudomonas sp. BL072 deacetyl-7-aminocephalosporanic acid + glutarate
-
 ?
glutaryl-7-aminocephalosporanic acid + H2O
-
 Pseudomonas sp. 7-aminocephalosporanate + glutarate
-
 ?
glutaryl-7-aminocephalosporanic acid + H2O
-
 Pseudomonas sp. BL072 7-aminocephalosporanate + glutarate
-
 ?
additional information the enzyme has transacylase activity 10times that of its hydrolytic activity Pseudomonas sp. ?
-
 ?
additional information the enzyme has transacylase activity 10times that of its hydrolytic activity Pseudomonas sp. BL072 ?
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 65000 + 1 * 24000 Pseudomonas sp.

Synonyms

Synonyms Comment Organism
glutaryl-7-aminocephalosporanic acid acylase
-
 Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Pseudomonas sp.

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas sp.
-
-
 8.5