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Literature summary for 3.5.1.9 extracted from
- Kynurenine formamidase. Purification and characterization of the adult chicken liver enzyme and immunochemical analyses of the enzyme of developing chicken (1974), J. Biol. Chem., 249, 4439-4444.
General Stability
| General Stability | Organism |
|---|---|
| sensitive to repeated freezing and thawing | Gallus gallus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | complete inhibition at 0.1 mM | Gallus gallus |  |
| Cu2+ | 21% inhibition at 0.1 mM | Gallus gallus | |
| Hg2+ | 57% inhibition at 0.1 mM | Gallus gallus | |
| p-nitrophenyldiethyl phosphoric acid | 92% inhibition at 10 mM | Gallus gallus | |
| phenylmethylsulfonyl fluoride | complete inhibition at 1 mM | Gallus gallus | |
| Urea | 75% inhibition at 1 M | Gallus gallus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.14 | - |
N-formyl-L-kynurenine | - |
Gallus gallus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
2 * 30000, SDS-PAGE | Gallus gallus |
| 59000 | - |
gel filtration | Gallus gallus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-formyl-L-kynurenine + H2O | Gallus gallus | - |
L-kynurenine + formate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gallus gallus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, chromatography techniques, preparative disc gel electrophoresis | Gallus gallus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Gallus gallus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 80.4 | - |
- |
Gallus gallus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 5°C, no inactivation during prolonged storage | Gallus gallus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-formyl-L-kynurenine + H2O | - |
Gallus gallus | L-kynurenine + formate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 30000, SDS-PAGE | Gallus gallus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Gallus gallus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.5 | active within | Gallus gallus |
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