Literature summary for 3.5.1.89 extracted from

Almani, P.G.N.; Sharifi, I.; Kazemi, B.; Babaei, Z.; Bandehpour, M.; Salari, S.; Dezaki, E.S.; Tohidi, F.; Mohammadi, M.A.
The role of GlcNAc-PI-de-N-acetylase gene by gene knockout throughhomologous recombination and its consequences on survival, growthand infectivity of Leishmania major in in vitro and in vivo conditions (2016), Acta Trop., 154, 63-72 .

Search for more literature for 3.5.1.89

Cloned(Commentary)

Cloned (Comment)	Organism
gene GPI12, cloning in Escherichia coli strain Top 10 and tranformation and recombinant expression in Leishmaia major null mutant cells	Leishmania major

Protein Variants

Protein Variants	Comment	Organism
additional information	two alleles of the GPI12 gene in Leishmania major are successfully removed enabling the generation of a null mutant, which supports the idea that GPI12 is not an essential gene for the growth and survival of Leishmania and the homozygous knockouts of Leishmania are able to survive. Generation of a mutant parasite that causes no damaged to the host	Leishmania major

Organism

Organism	UniProt	Comment	Textmining
Leishmania major	Q8I8A4	isolated from infected BALB/c mice	-
Leishmania major 75	Q8I8A4	isolated from infected BALB/c mice	-
Leishmania major ER	Q8I8A4	isolated from infected BALB/c mice	-
Leishmania major IR	Q8I8A4	isolated from infected BALB/c mice	-
Leishmania major MRHO	Q8I8A4	isolated from infected BALB/c mice	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
amastigote	-	Leishmania major	-
promastigote	-	Leishmania major	-

Synonyms

Synonyms	Comment	Organism
GlcNAc-PI-de-N-acetylase	-	Leishmania major
GPI12	-	Leishmania major

General Information

General Information	Comment	Organism
malfunction	an in vitro study using murine MQ cell line J774 shows that by the use of recombinant parasites the entry rate and infecting ability is decreased. The mutant parasites are unable to produce skin lesions even six months post-infection in the BALB/c mice	Leishmania major
physiological function	GPI12 is not an essential gene for the growth and survival of Leishmania and the homozygous knockouts of Leishmania are able to survive. Stationary phase promastigotes are used for in vitro cell line J774 macrophage infectivity test also for in vivo infection	Leishmania major

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Release 2023.1 (January 2023)