Cloned (Comment) | Organism |
---|---|
the cytoplasmic domain MBPEhDELTATMPIG-L is expressed in Escherichia coli | Entamoeba histolytica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | treatment with EDTA consistently causes up to 2% reduction in activity, the activity is not significantly affected by treatment with 1,10-phenanthroline | Entamoeba histolytica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00195 | - |
N-acetyl-D-glucosaminylphosphatidylinositol | in the absence of metal ions, in 50 mM acetate buffer at pH 5.5 and 37°C | Entamoeba histolytica | |
0.00205 | - |
N-acetyl-D-glucosaminylphosphatidylinositol | in the presence of 5 mM Mn2+, in 50 mM acetate buffer at pH 5.5 and 37°C | Entamoeba histolytica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | the de-N-acetylase activity of the cyctoplasmic domain MBPEhDELTATMPIG-L is 2fold higher in the presence of 5 mM Co2+ | Entamoeba histolytica | |
Mg2+ | the de-N-acetylase activity of the cyctoplasmic domain MBPEhDELTATMPIG-L is 2fold higher in the presence of 5 mM Mg2+ | Entamoeba histolytica | |
Mn2+ | the de-N-acetylase activity of the cyctoplasmic domain MBPEhDELTATMPIG-L is 2fold higher in the presence of 5 mM Mn2+ | Entamoeba histolytica | |
additional information | 5 mM Zn2+ does not stimulate the de-N-acetylase activity of the cyctoplasmic domain MBPEhDELTATMPIG-L | Entamoeba histolytica | |
additional information | the protein is active even in the absence of metal. The metal ion does not directly participate in ligand binding or recognition but is important for the catalytic efficiency of the enzymatic site | Entamoeba histolytica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
x * 66000, cytoplasmic domain MBPEhDELTATMPIG-L, SDS-PAGE | Entamoeba histolytica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-Acetyl-D-glucosaminylphosphatidylinositol + H2O | Entamoeba histolytica | - |
D-Glucosaminylphosphatidylinositol + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Entamoeba histolytica | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-Acetyl-D-glucosaminylphosphatidylinositol + H2O | - |
Entamoeba histolytica | D-Glucosaminylphosphatidylinositol + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 66000, cytoplasmic domain MBPEhDELTATMPIG-L, SDS-PAGE | Entamoeba histolytica |
Synonyms | Comment | Organism |
---|---|---|
GlcNAc-PI-de-N-acetylase | - |
Entamoeba histolytica |
N-acetyl-D-glucosaminylphosphatidylinositol de-N-acetylase | - |
Entamoeba histolytica |
PIG-L protein | - |
Entamoeba histolytica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Entamoeba histolytica |