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Literature summary for 3.5.1.89 extracted from

  • Milne, K.G.; Field, R.A.; Masterson, W.J.; Cooaz, S.; Brimacombe, J.S.; Ferguson, M.A.J.
    Partial purification and characterization of the N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase of glycosylphosphatidylinositol anchor biosynthesis in African trypanosomes (1994), J. Biol. Chem., 269, 16403-16408.
    View publication on PubMed
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General Stability

General Stability Organism
10-50% v/v glycerol increases stability of solubilized enzyme Trypanosoma sp.
Solubilized enzyme loses activity upon freeze-thawing Trypanosoma sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00015
-
 N-acetyl-D-glucosaminylphosphatidylinositol
-
 Trypanosoma sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Trypanosoma sp. 16020
-

Organism

Organism UniProt Comment Textmining
Trypanosoma sp.
-
 variant MITat 1.4
-

Purification (Commentary)

Purification (Comment) Organism
variant MITat 1.4, partial Trypanosoma sp.

Source Tissue

Source Tissue Comment Organism Textmining
bloodstream form of Trypanosoma Trypanosoma sp.
-

Storage Stability

Storage Stability Organism
Highly purified preparation is extremely labile, even when stored in 50% glycerol Trypanosoma sp.
Solubilized enzyme loses activity upon prolonged storage at 4°C Trypanosoma sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-Acetyl-D-glucosaminylphosphatidylinositol + H2O analogues lacking the glycerophosphate component are not recognized Trypanosoma sp. D-Glucosaminylphosphatidylinositol + acetate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 solubilized enzyme Trypanosoma sp.

pH Range

pH Minimum pH Maximum Comment Organism
6.4 8 90% of activity maximum at pH 6.4 and 8.0, solubilized enzyme Trypanosoma sp.