Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS cells | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
PDF of Escherichia coli with Ni2+ replacing the native Fe2+, hanging drop vapour diffusion method, using 20.5% (w/v) PEG 4000, 0.1 M sodium acetate, pH 4.0 | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ni2+ | addition of 1 mM Ni2+ increases the PDF activity by 70%, with 20 mM Ni2+, the activity decreases by 23% compared with the low Ni2+ concentration | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | enzymatic activity of Escherichia coli PDF is retained after replacement of the active-site Fe2+ ion with Co2+ | Escherichia coli | |
Fe2+ | the native enzyme contains iron | Escherichia coli | |
Ni2+ | enzymatic activity of Escherichia coli PDF is retained after replacement of the active-site Fe2+ ion with Ni2+, the activity of the protein is not affected by Ni2+ concentrations up to 0.1 mM | Escherichia coli | |
Zn2+ | the Zn2+-PDF complex shows 500fold decreased activity | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A6K3 | - |
- |
Purification (Comment) | Organism |
---|---|
Poros 20 MC column chromatography and Poros 20 HQ column chromatography | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
- |
Escherichia coli |