Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.1.88 extracted from

  • Yen, N.T.; Bogdanovic, X.; Palm, G.J.; Kuehl, O.; Hinrichs, W.
    Structure of the Ni(II) complex of Escherichia coli peptide deformylase and suggestions on deformylase activities depending on different metal(II) centres (2009), J. Biol. Inorg. Chem., 15, 195-201.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
PDF of Escherichia coli with Ni2+ replacing the native Fe2+, hanging drop vapour diffusion method, using 20.5% (w/v) PEG 4000, 0.1 M sodium acetate, pH 4.0 Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Ni2+ addition of 1 mM Ni2+ increases the PDF activity by 70%, with 20 mM Ni2+, the activity decreases by 23% compared with the low Ni2+ concentration Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ enzymatic activity of Escherichia coli PDF is retained after replacement of the active-site Fe2+ ion with Co2+ Escherichia coli
Fe2+ the native enzyme contains iron Escherichia coli
Ni2+ enzymatic activity of Escherichia coli PDF is retained after replacement of the active-site Fe2+ ion with Ni2+, the activity of the protein is not affected by Ni2+ concentrations up to 0.1 mM Escherichia coli
Zn2+ the Zn2+-PDF complex shows 500fold decreased activity Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A6K3
-
-

Purification (Commentary)

Purification (Comment) Organism
Poros 20 MC column chromatography and Poros 20 HQ column chromatography Escherichia coli

Synonyms

Synonyms Comment Organism
PDF
-
Escherichia coli