Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
E133A | 10000000fold reduction in activity | Escherichia coli |
E133C | mutant fails to produce soluble protein | Escherichia coli |
E133D | modest reduction in activity, less than 10fold for most of the substrates tested | Escherichia coli |
E133Q | mutant fails to produce soluble protein | Escherichia coli |
General Stability | Organism |
---|---|
enhanced stability by binding of Co2+ | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(S)-2-O-(H-phosphonoxy)-L-caproyl-L-Leu-p-nitroanilide | similar inhibition of Fe2+- and Co2+-bound enzyme | Escherichia coli | |
1,10-phenanthroline | similar inhibition of Fe2+- and Co2+-bound enzyme | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
N-formyl-beta-thiaphenylalanyl-lysyl-p-nitroanilide | recombinant mutant E133D Co2+-bound enzyme | Escherichia coli | |
0.018 | - |
N-formylmethionyl-Leu-p-nitroanilide | recombinant Co2+-bound enzyme | Escherichia coli | |
0.02 | - |
N-formylmethionyl-Leu-p-nitroanilide | recombinant Fe2+-bound enzyme | Escherichia coli | |
0.027 | - |
N-formyl-beta-thiaphenylalanyl-Lys-p-nitroanilide | recombinant Fe2+-bound enzyme | Escherichia coli | |
0.028 | - |
N-formylmethionyl-Leu-p-nitroanilide | recombinant mutant E133D Co2+-bound enzyme | Escherichia coli | |
0.03 | - |
N-formyl-beta-thiaphenylalanyl-Lys-p-nitroanilide | recombinant Co2+-bound enzyme | Escherichia coli | |
2.64 | - |
N-formyl-Met-Leu-NH2 | recombinant Fe2+-bound enzyme | Escherichia coli | |
7.36 | - |
N-formylmethionylleucyl-amide | recombinant Co2+-bound enzyme | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | Co2+-bound enzyme, highly stable | Escherichia coli | |
Fe2+ | natural metal, bound to active site | Escherichia coli | |
Fe2+ | sensitive to reactive oxygen | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Oxidation Stability | Organism |
---|---|
sensitive to intracellular reactive oxygen | Escherichia coli |
Purification (Comment) | Organism |
---|---|
to homogeneity, recombinant Co2+-bound enzyme | Escherichia coli |
Storage Stability | Organism |
---|---|
4°C, or at room temperature, stable for hours to days | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formyl-L-Met-Leu-p-nitroanilide + H2O | - |
Escherichia coli | formate + Met-Leu-p-nitroanilide | - |
? | |
N-formyl-beta-thiaphenylalanyl-Lys-p-nitroanilide + H2O | - |
Escherichia coli | formate + beta-thiaphenylalanyl-Lys-p-nitroanilide | - |
? | |
N-formyl-beta-thiaphenylalanyl-peptide + H2O | - |
Escherichia coli | formate + beta-thiaphenylalanyl-peptide | - |
? | |
N-formyl-Met-Leu-NH2 + H2O | - |
Escherichia coli | formate + Met-Leu-NH2 | - |
? | |
N-formyl-Met-Leu-p-nitroanilide + H2O | - |
Escherichia coli | formate + Met-Leu-p-nitroanilide | - |
? | |
N-formylmethionylleucyl-amide + H2O | - |
Escherichia coli | formate + methionylleucyl-amide | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.2 | - |
N-formylmethionylleucyl-p-nitroanilide | recombinant mutant E133D Co2+-bound enzyme | Escherichia coli | |
19 | - |
N-formyl-Met-Leu-p-nitroanilide | recombinant Co2+-bound enzyme | Escherichia coli | |
70 | - |
N-formylmethionylleucyl-p-nitroanilide | recombinant Fe2+-bound enzyme | Escherichia coli | |
1280 | - |
N-formyl-Met-Leu-NH2 | recombinant Co2+-bound enzyme | Escherichia coli | |
1320 | - |
N-formyl-Met-Leu-NH2 | recombinant Fe2+-bound enzyme | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
mutant E133D Co2+-bound enzyme | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 11.6 | optimal activity within, Fe2+- and Co2+-bound enzyme | Escherichia coli |
7.5 | 9.5 | mutant E133D Co2+-bound enzyme | Escherichia coli |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
loss of activity below | Escherichia coli |