Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Pseudomonas sp. | |
Zn2+ | - |
Pseudomonas sp. |
Cloned (Comment) | Organism |
---|---|
NCC amidohydrolase gene is cloned into DH5alpha and pet15b vectors for protein expression in Escherichia coli | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Pseudomonas sp. | |
Hg2+ | - |
Pseudomonas sp. | |
iodoacetic acid | IAA | Pseudomonas sp. | |
N-ethyl-maleimide | NEM | Pseudomonas sp. | |
p-chloromercuribenzoic acid | PCMB | Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | - |
Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
monomer, 12,5% SDS-PAGE | Pseudomonas sp. |
170000 | - |
native enzyme, tetramer, estimated by Sephacryl S-300 gel filtration | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-carbamoyl-L-cysteine + H2O | Pseudomonas sp. | - |
L-cysteine + CO2 + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
anion-exchange chromatography, hydrophobic interaction chromatography hydroxyapatite chromatography used for purification of native expressed protein | Pseudomonas sp. |
Renatured (Comment) | Organism |
---|---|
protein from inclusion bodies is solubilized, recovered and purified by metal affinity chromatography | Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15.9 | - |
protein recovered from inclusion bodies | Pseudomonas sp. |
23.2 | - |
- |
Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-carbamoyl-DL-aspartic acid + H2O | relative activity 7.81% | Pseudomonas sp. | DL-aspartic acid + CO2 + NH3 | - |
? | |
N-carbamoyl-DL-cysteine + H2O | relative activity 119% | Pseudomonas sp. | DL-cysteine + CO2 + NH3 | - |
? | |
N-carbamoyl-DL-serine + H2O | relative activity 37.8% | Pseudomonas sp. | DL-serine + CO2 + NH3 | - |
? | |
N-carbamoyl-glycine + H2O | relative activity 72.4% | Pseudomonas sp. | glycine + CO2 + NH3 | - |
? | |
N-carbamoyl-L-alanine + H2O | relative activity 41.2% | Pseudomonas sp. | L-alanine + CO2 + NH3 | - |
? | |
N-carbamoyl-L-cysteine + H2O | - |
Pseudomonas sp. | L-cysteine + CO2 + NH3 | - |
? | |
N-carbamoyl-L-cysteine + H2O | relative activity 100% | Pseudomonas sp. | L-cysteine + CO2 + NH3 | - |
? | |
N-carbamoyl-L-methionine + H2O | relative activity 44.4% | Pseudomonas sp. | L-methionine + CO2 + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Pseudomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
N-carbamoyl-L-amino-acid amidohydrolase | - |
Pseudomonas sp. |
N-carbamoyl-L-amino-acid hydrolase | - |
Pseudomonas sp. |
N-carbamoyl-L-cysteine-acid amidohydrolase | - |
Pseudomonas sp. |
NCC amidohydrolase | - |
Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
activity assay | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
activity assay | Pseudomonas sp. |