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Literature summary for 3.5.1.87 extracted from

  • Ohmachi, T.; Narita, M.; Kawata, M.; Bizen, A.; Tamura, Y.; Asada, Y.
    A novel N-carbamoyl-L-amino acid amidohydrolase of Pseudomonas sp. strain ON-4a: purification and characterization of N-carbamoyl-L-cysteine amidohydrolase expressed in Escherichia coli (2004), Appl. Microbiol. Biotechnol., 65, 686-693.
    View publication on PubMed
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Activating Compound

Activating Compound Comment Organism Structure
Mg2+
-
 Pseudomonas sp.
Zn2+
-
 Pseudomonas sp.

Cloned(Commentary)

Cloned (Comment) Organism
NCC amidohydrolase gene is cloned into DH5alpha and pet15b vectors for protein expression in Escherichia coli Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Pseudomonas sp.
Hg2+
-
 Pseudomonas sp.
iodoacetic acid IAA Pseudomonas sp.
N-ethyl-maleimide NEM Pseudomonas sp.
p-chloromercuribenzoic acid PCMB Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+
-
 Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 monomer, 12,5% SDS-PAGE Pseudomonas sp.
170000
-
 native enzyme, tetramer, estimated by Sephacryl S-300 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-carbamoyl-L-cysteine + H2O Pseudomonas sp.
-
 L-cysteine + CO2 + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
anion-exchange chromatography, hydrophobic interaction chromatography hydroxyapatite chromatography used for purification of native expressed protein Pseudomonas sp.

Renatured (Commentary)

Renatured (Comment) Organism
protein from inclusion bodies is solubilized, recovered and purified by metal affinity chromatography Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15.9
-
 protein recovered from inclusion bodies Pseudomonas sp.
23.2
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-carbamoyl-DL-aspartic acid + H2O relative activity 7.81% Pseudomonas sp. DL-aspartic acid + CO2 + NH3
-
 ?
N-carbamoyl-DL-cysteine + H2O relative activity 119% Pseudomonas sp. DL-cysteine + CO2 + NH3
-
 ?
N-carbamoyl-DL-serine + H2O relative activity 37.8% Pseudomonas sp. DL-serine + CO2 + NH3
-
 ?
N-carbamoyl-glycine + H2O relative activity 72.4% Pseudomonas sp. glycine + CO2 + NH3
-
 ?
N-carbamoyl-L-alanine + H2O relative activity 41.2% Pseudomonas sp. L-alanine + CO2 + NH3
-
 ?
N-carbamoyl-L-cysteine + H2O
-
 Pseudomonas sp. L-cysteine + CO2 + NH3
-
 ?
N-carbamoyl-L-cysteine + H2O relative activity 100% Pseudomonas sp. L-cysteine + CO2 + NH3
-
 ?
N-carbamoyl-L-methionine + H2O relative activity 44.4% Pseudomonas sp. L-methionine + CO2 + NH3
-
 ?

Subunits

Subunits Comment Organism
tetramer
-
 Pseudomonas sp.

Synonyms

Synonyms Comment Organism
N-carbamoyl-L-amino-acid amidohydrolase
-
 Pseudomonas sp.
N-carbamoyl-L-amino-acid hydrolase
-
 Pseudomonas sp.
N-carbamoyl-L-cysteine-acid amidohydrolase
-
 Pseudomonas sp.
NCC amidohydrolase
-
 Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 activity assay Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 activity assay Pseudomonas sp.