Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Paenarthrobacter aurescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
2 * 44000, SDS-PAGE | Paenarthrobacter aurescens |
93000 | - |
gel filtration | Paenarthrobacter aurescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenarthrobacter aurescens | - |
strain DSM 3747 | - |
Purification (Comment) | Organism |
---|---|
streamline DEAE, Mono Q | Paenarthrobacter aurescens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5 | - |
- |
Paenarthrobacter aurescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-carbamoyl-DL-phenylalanine + H2O | enzyme seems to be selective for aromatic amino acids | Paenarthrobacter aurescens | L-phenylalanine + CO2 + NH3 | - |
? | |
N-carbamoyl-L-methionine + H2O | L-stereospecific enzyme | Paenarthrobacter aurescens | L-methionine + CO2 + NH3 | - |
? | |
N-carbamoyl-L-thienylalanine + H2O | - |
Paenarthrobacter aurescens | L-thienylalanine + CO2 + NH3 | - |
? | |
N-carbamoyl-L-tryptophan + H2O | - |
Paenarthrobacter aurescens | L-tryptophan + CO2 + NH3 | - |
? | |
N-carbamoyl-L-tyrosine + H2O | - |
Paenarthrobacter aurescens | L-tyrosine + CO2 + NH3 | - |
? | |
N-formyl-DL-tryptophan + H2O | - |
Paenarthrobacter aurescens | L-tryptophan + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 44000, SDS-PAGE | Paenarthrobacter aurescens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Paenarthrobacter aurescens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 55 | no loss of activity after 6 d at 37°C, 50% loss of activity after 2 h at 50°C | Paenarthrobacter aurescens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
N-carbamoyl-L-methionine | - |
Paenarthrobacter aurescens | |
5 | - |
N-formyl-DL-tryptophan | - |
Paenarthrobacter aurescens | |
5.1 | - |
N-carbamoyl-L-tryptophan | - |
Paenarthrobacter aurescens | |
6.5 | - |
N-carbamoyl-L-tyrosine | - |
Paenarthrobacter aurescens | |
16.1 | - |
N-carbamoyl-L-thienylalanine | - |
Paenarthrobacter aurescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | - |
Paenarthrobacter aurescens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 11 | enzyme activity significantly decreases below, enzyme is highly active up to pH 11.0 | Paenarthrobacter aurescens |