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Literature summary for 3.5.1.87 extracted from

  • Ogawa, J.; Miyake, H.; Shimizu, S.
    Purification and characterization of N-carbamoyl-L-amino acid amidohydrolase with broad substrate specificity from Alcaligenes xylosoxidans (1995), Appl. Microbiol. Biotechnol., 43, 1039-1043.
    View publication on PubMed
Search for more literature for 3.5.1.87

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoic acid) 2 mM, 100% inhibition Achromobacter xylosoxidans
8-hydroxyquinoline 2 mM, 65% inhibition Achromobacter xylosoxidans
alpha,alpha'-dipyridyl 2.5 mM, 26% inhibition Achromobacter xylosoxidans
Cd2+ 2 mM, 86% inhibition Achromobacter xylosoxidans
Cu2+ 2 mM, 85% inhibition Achromobacter xylosoxidans
diisopropyl fluorophosphate 5 mM, 18% inhibition Achromobacter xylosoxidans
EDTA 10 mM, 22% inhibition Achromobacter xylosoxidans
Hg2+ 2 mM, 100% inhibition Achromobacter xylosoxidans
iodoacetate 2 mM, 43% inhibitiion Achromobacter xylosoxidans
additional information not inhibited by 5 mM NaCN, NaF, NaN3, NH2OH, semicarbazide, (NH4)2SO4, NaCO3, N-carbamoyl-D-valine, N-carbamoyl-D-alanine or L-alanine Achromobacter xylosoxidans
N-ethylmaleimide 2 mM, 100% inhibitiion Achromobacter xylosoxidans
NaAsO2 5 mM, 20% inhibition Achromobacter xylosoxidans
o-phenanthroline 10 mM, 47% inhibition Achromobacter xylosoxidans
p-chloromercuribenzoic acid 2 mM, 100% inhibitiion Achromobacter xylosoxidans
phenylhydrazine 5 mM, 48% inhibition Achromobacter xylosoxidans
phenylmethylsulfonyl fluoride 5 mM, 31% inhibition Achromobacter xylosoxidans
sodium propionate 10 mM, 20% inhibition, competitive inhibition of N-carbamoyl-L-alanine hydrolysis Achromobacter xylosoxidans
Zn2+ 2 mM, 85% inhibition Achromobacter xylosoxidans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.31
-
 N-carbamoyl-L-isoleucine
-
 Achromobacter xylosoxidans
0.4
-
 N-carbamoyl-L-valine
-
 Achromobacter xylosoxidans
0.85
-
 N-carbamoyl-DL-2-aminohexanoic acid
-
 Achromobacter xylosoxidans
0.86
-
 N-carbamoyl-L-leucine
-
 Achromobacter xylosoxidans
0.92
-
 N-carbamoyl-L-phenylalanine
-
 Achromobacter xylosoxidans
1.5
-
 N-carbamoyl-L-asparagine
-
 Achromobacter xylosoxidans
3
-
 N-carbamoyl-L-alanine
-
 Achromobacter xylosoxidans
3.2
-
 N-carbamoyl-DL-methionine
-
 Achromobacter xylosoxidans
6.6
-
 N-Carbamoylglycine
-
 Achromobacter xylosoxidans
10
-
 N-carbamoyl-DL-serine
-
 Achromobacter xylosoxidans
16
-
 N-formyl-DL-alanine
-
 Achromobacter xylosoxidans

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 0.5 mM, 195% activation of 8-hydroxyquinoline treated enzyme with 38% of initial Achromobacter xylosoxidans
Mn2+ 0.3 mM, 360% activation of 8-hydroxyquinoline treated enzyme with 38% of initial activity Achromobacter xylosoxidans
Ni2+ 0.3 mM, 200% activation of 8-hydroxyquinoline treated enzyme with 38% of initial activity Achromobacter xylosoxidans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 2 * 65000, SDS-PAGE Achromobacter xylosoxidans
134000
-
 gel filtration Achromobacter xylosoxidans

Organism

Organism UniProt Comment Textmining
Achromobacter xylosoxidans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sephacel, ammonium sulfate, phenyl-Sepharose CL-4B, Mono Q, Superose 12 HR Achromobacter xylosoxidans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.22
-
-
 Achromobacter xylosoxidans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-carbamoyl-DL-2-aminohexanoic acid + H2O 24% of activity with N-carbamoyl-DL-alanine Achromobacter xylosoxidans 2-aminohexanoic acid + CO2 + NH3
-
 ?
N-carbamoyl-DL-alanine + H2O
-
 Achromobacter xylosoxidans L-alanine + CO2 + NH3
-
 ?
N-carbamoyl-DL-methionine + H2O
-
 Achromobacter xylosoxidans L-methionine + CO2 + NH3
-
 ?
N-carbamoyl-DL-phenylalanine + H2O 4.5% of activity with N-carbamoyl-L-alanine Achromobacter xylosoxidans L-phenylalanine + CO2 + NH3
-
 ?
N-carbamoyl-DL-serine + H2O 19% of activity with N-carbamoyl-L-alanine Achromobacter xylosoxidans L-serine + CO2 + NH3
-
 ?
N-carbamoyl-DL-threonine + H2O 8.8% of activity with N-carbamoyl-L-alanine Achromobacter xylosoxidans L-threonine + CO2 + NH3
-
 ?
N-carbamoyl-DL-valine + H2O 28% of activity with N-carbamoyl-L-alanine Achromobacter xylosoxidans L-valine + CO2 + NH3
-
 ?
N-carbamoyl-glycine + H2O 75% of activity with N-carbamoyl-L-alanine Achromobacter xylosoxidans glycine + CO2 + NH3
-
 ?
N-carbamoyl-L-alanine + H2O
-
 Achromobacter xylosoxidans L-alanine + CO2 + NH3
-
 ?
N-carbamoyl-L-asparagine + H2O 64% of activity with N-carbamoyl-L-alanine Achromobacter xylosoxidans L-asparagine + CO2 + NH3
-
 ?
N-carbamoyl-L-isoleucine + H2O 5.3% of activity with N-carbamoyl-L-alanine Achromobacter xylosoxidans L-isoleucine + CO2 + NH3
-
 ?
N-carbamoyl-L-leucine + H2O
-
 Achromobacter xylosoxidans L-leucine + CO2 + NH3
-
 ?
N-carbamoyl-L-methionine + H2O 12% of activity with N-carbamoyl-L-alanine Achromobacter xylosoxidans L-methionine + CO2 + NH3
-
 ?
N-carbamoyl-L-methionine + H2O L-stereospecific enzyme Achromobacter xylosoxidans L-methionine + CO2 + NH3
-
 ?
N-carbamoyl-L-phenylalanine + H2O
-
 Achromobacter xylosoxidans L-phenylalanine + CO2 + NH3
-
 ?
N-carbamoyl-L-valine + H2O
-
 Achromobacter xylosoxidans L-valine + CO2 + NH3
-
 ?
N-formyl-DL-alanine + H2O 13% of activity with N-carbamoyl-DL-alanine Achromobacter xylosoxidans L-alanine + CO2
-
 ?
N-formyl-DL-leucine + H2O 5.2% of activity with N-carbamoyl-DL-alanine Achromobacter xylosoxidans L-leucine + CO2
-
 ?
N-formyl-DL-methionine + H2O 5.4% of activity with N-carbamoyl-DL-alanine Achromobacter xylosoxidans L-methionine + CO2
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 65000, SDS-PAGE Achromobacter xylosoxidans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Achromobacter xylosoxidans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 35 no loss of activity after 30 min at pH 7.4, 30% activity lost after 30 min at 35°C Achromobacter xylosoxidans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.3 N-carbamoyl-L-valine hydrolysis Achromobacter xylosoxidans

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9.5 80% of initial activity after 30 min and 30°C Achromobacter xylosoxidans