Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli both wild type and His6-tagged protein | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
K100A | no enzymic activity | Pseudomonas putida |
S180A | significant decrease in kcat value | Pseudomonas putida |
S181A | significant decrease in kcat value | Pseudomonas putida |
S204A | no enzymic activity | Pseudomonas putida |
S217A | very little enzymic activity | Pseudomonas putida |
S218A | very little enzymic activity | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-phenyl-1,1,1-trifluoro-2-propanone | competitive | Pseudomonas putida | |
3-phenyl-1-chloro-2-propanone | competitive | Pseudomonas putida | |
PMSF | 1 mM, 80% inhibition | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0038 | - |
2-phenylacetamide | pH 7.5, 30°C | Pseudomonas putida | |
0.009 | - |
(S)-mandelamide | pH 7.5, 30°C, mutant S181A | Pseudomonas putida | |
0.02 | - |
(S)-mandelamide | pH 7.5, 30°C | Pseudomonas putida | |
0.032 | - |
N-methyl phenylacetamide | pH 7.5, 30°C | Pseudomonas putida | |
0.033 | - |
(R)-mandelamide | pH 7.5, 30°C | Pseudomonas putida | |
0.049 | - |
3-phenylpropionamide | pH 7.5, 30°C | Pseudomonas putida | |
0.059 | - |
(R)-mandelamide | pH 7.5, 30°C, mutant S181A | Pseudomonas putida | |
0.17 | - |
(R,S)-methyl mandelate | pH 7.5, 30°C | Pseudomonas putida | |
0.288 | - |
hexanoamide | pH 7.5, 30°C | Pseudomonas putida | |
0.296 | - |
(S)-mandelamide | pH 7.5, 30°C, mutant S180A | Pseudomonas putida | |
0.871 | - |
(R)-mandelamide | pH 7.5, 30°C, mutant S180A | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53800 | - |
x * 54000, SDS-PAGE, x * 53800, mass spectrometry | Pseudomonas putida |
54000 | - |
x * 54000, SDS-PAGE, x * 53800, mass spectrometry | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | Q84DC4 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Pseudomonas putida |
Storage Stability | Organism |
---|---|
-20°C, no loss of activtiy during several freeze-thaw cycles | Pseudomonas putida |
4°C, phosphate buffer pH 7.8, 1 mM EDTA, stable for at least 1 month | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-mandelamide + H2O | - |
Pseudomonas putida | (R)-mandelate + NH3 | - |
? | |
(R,S)-methyl mandelate + H2O | - |
Pseudomonas putida | ? | - |
? | |
(S)-mandelamide + H2O | - |
Pseudomonas putida | (S)-mandelate + NH3 | - |
? | |
2-phenylacetamide + H2O | - |
Pseudomonas putida | ? | - |
? | |
3-phenylpropionamide + H2O | - |
Pseudomonas putida | ? | - |
? | |
hexanoamide + H2O | - |
Pseudomonas putida | ? | - |
? | |
N-methyl phenylacetamide + H2O | - |
Pseudomonas putida | ? | - |
? | |
R,S-mandelamide + H2O | - |
Pseudomonas putida | mandelic acid + ammonia | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 54000, SDS-PAGE, x * 53800, mass spectrometry | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
MAH | - |
Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Pseudomonas putida |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
3-phenyl-1,1,1-trifluoro-2-propanone | pH 7.5, 30°C | Pseudomonas putida | |
0.13 | - |
3-phenyl-1-chloro-2-propanone | pH 7.5, 30°C | Pseudomonas putida |