Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli DH5alpha cells | Rhizobium leguminosarum bv. viciae |
General Stability | Organism |
---|---|
potassium phosphate buffer yields optimal catalytic rates | Rhizobium leguminosarum bv. viciae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-ethylmaleimide | more than 90% loss of activity at 0.05 mM; more than 90% loss of activity at 0.5 mM | Rhizobium leguminosarum bv. viciae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.023 | - |
Biuret | in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C | Rhizobium leguminosarum bv. viciae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the addition of ZnSO4 or CoSO4 (0.05 mM and 1 mM) to enzyme assays gives only nominal increases in activity. No metal cofactors are detected | Rhizobium leguminosarum bv. viciae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27121 | - |
x * 27121, calculated from amino acid sequence | Rhizobium leguminosarum bv. viciae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
biuret + H2O | Rhizobium leguminosarum bv. viciae | the enzyme acts uniquely on biuret physiologically | allophanic acid + NH3 | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizobium leguminosarum bv. viciae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
nickel affinity column chromatography | Rhizobium leguminosarum bv. viciae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.02 | - |
below 0.02 micromol/min/mg, using (E)-diazene-1,2-dicarboxamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C | Rhizobium leguminosarum bv. viciae |
0.02 | - |
below 0.02 micromol/min/mg, using 1,1-dimethylurea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C | Rhizobium leguminosarum bv. viciae |
0.02 | - |
below 0.02 micromol/min/mg, using 1-carbamimidoylthiourea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C | Rhizobium leguminosarum bv. viciae |
0.02 | - |
below 0.02 micromol/min/mg, using 1-ethylurea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C | Rhizobium leguminosarum bv. viciae |
0.02 | - |
below 0.02 micromol/min/mg, using 1-hydroxyurea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C | Rhizobium leguminosarum bv. viciae |
0.02 | - |
below 0.02 micromol/min/mg, using 1-methylthiourea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C | Rhizobium leguminosarum bv. viciae |
0.02 | - |
below 0.02 micromol/min/mg, using 1-methylurea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C | Rhizobium leguminosarum bv. viciae |
0.02 | - |
below 0.02 micromol/min/mg, using 1-prop-2-en-1-ylurea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C | Rhizobium leguminosarum bv. viciae |
0.02 | - |
below 0.02 micromol/min/mg, using 2-cyanoacetamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C | Rhizobium leguminosarum bv. viciae |
0.02 | - |
below 0.02 micromol/min/mg, using 2-methylpropanamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C | Rhizobium leguminosarum bv. viciae |
0.02 | - |
below 0.02 micromol/min/mg, using 3-oxobutanamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C | Rhizobium leguminosarum bv. viciae |
0.02 | - |
below 0.02 micromol/min/mg, using acetamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C | Rhizobium leguminosarum bv. viciae |
0.02 | - |
below 0.02 micromol/min/mg, using butanediamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C | Rhizobium leguminosarum bv. viciae |
0.02 | - |
below 0.02 micromol/min/mg, using carbamoylcarbamate as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C | Rhizobium leguminosarum bv. viciae |
0.02 | - |
below 0.02 micromol/min/mg, using dicarbonodithioimidic diamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C | Rhizobium leguminosarum bv. viciae |
0.02 | - |
below 0.02 micromol/min/mg, using ethanediamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C | Rhizobium leguminosarum bv. viciae |
0.02 | - |
below 0.02 micromol/min/mg, using ethanethioamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C | Rhizobium leguminosarum bv. viciae |
0.02 | - |
below 0.02 micromol/min/mg, using ethyl acetate as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C | Rhizobium leguminosarum bv. viciae |
0.02 | - |
below 0.02 micromol/min/mg, using ethyl carbamoylcarbamate as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C | Rhizobium leguminosarum bv. viciae |
0.02 | - |
below 0.02 micromol/min/mg, using formamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C | Rhizobium leguminosarum bv. viciae |
0.02 | - |
below 0.02 micromol/min/mg, using hexanediamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C | Rhizobium leguminosarum bv. viciae |
0.02 | - |
below 0.02 micromol/min/mg, using hydrazinecarbothioamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C | Rhizobium leguminosarum bv. viciae |
0.02 | - |
below 0.02 micromol/min/mg, using hydrazinecarboxamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C | Rhizobium leguminosarum bv. viciae |
0.02 | - |
below 0.02 micromol/min/mg, using N-carbamothioylacetamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C | Rhizobium leguminosarum bv. viciae |
0.02 | - |
below 0.02 micromol/min/mg, using N-hydroxydicarbonimidic diamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C | Rhizobium leguminosarum bv. viciae |
0.02 | - |
below 0.02 micromol/min/mg, using N-nitrodicarbonimidic diamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C | Rhizobium leguminosarum bv. viciae |
0.02 | - |
below 0.02 micromol/min/mg, using prop-2-enamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C | Rhizobium leguminosarum bv. viciae |
0.02 | - |
below 0.02 micromol/min/mg, using propanediamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C | Rhizobium leguminosarum bv. viciae |
0.02 | - |
below 0.02 micromol/min/mg, using sulfuric diamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C | Rhizobium leguminosarum bv. viciae |
0.02 | - |
below 0.02 micromol/min/mg, using thiourea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C | Rhizobium leguminosarum bv. viciae |
0.02 | - |
below 0.02 micromol/min/mg, using urea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C | Rhizobium leguminosarum bv. viciae |
0.04 | - |
using N-carbamoylacetamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C | Rhizobium leguminosarum bv. viciae |
0.17 | - |
using 1-formylurea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C | Rhizobium leguminosarum bv. viciae |
6.08 | - |
using dicarbonimidic diamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C | Rhizobium leguminosarum bv. viciae |
8.9 | - |
using biuret as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C | Rhizobium leguminosarum bv. viciae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E)-diazene-1,2-dicarboxamide + H2O | low specific activity | Rhizobium leguminosarum bv. viciae | ? | - |
? | |
1,1-dimethylurea + H2O | low specific activity | Rhizobium leguminosarum bv. viciae | ? | - |
? | |
1-carbamimidoylthiourea + H2O | low specific activity | Rhizobium leguminosarum bv. viciae | ? | - |
? | |
1-ethylurea + H2O | low specific activity | Rhizobium leguminosarum bv. viciae | ? | - |
? | |
1-formylurea + H2O | - |
Rhizobium leguminosarum bv. viciae | ? | - |
? | |
1-formylurea + H2O | low specific activity | Rhizobium leguminosarum bv. viciae | ? | - |
? | |
1-hydroxyurea + H2O | low specific activity | Rhizobium leguminosarum bv. viciae | ? | - |
? | |
1-methylthiourea + H2O | low specific activity | Rhizobium leguminosarum bv. viciae | ? | - |
? | |
1-methylurea + H2O | low specific activity | Rhizobium leguminosarum bv. viciae | ? | - |
? | |
1-prop-2-en-1-ylurea + H2O | low specific activity | Rhizobium leguminosarum bv. viciae | ? | - |
? | |
2-cyanoacetamide + H2O | low specific activity | Rhizobium leguminosarum bv. viciae | ? | - |
? | |
2-methylpropanamide + H2O | low specific activity | Rhizobium leguminosarum bv. viciae | ? | - |
? | |
3-oxobutanamide + H2O | low specific activity | Rhizobium leguminosarum bv. viciae | ? | - |
? | |
acetamide + H2O | low specific activity | Rhizobium leguminosarum bv. viciae | ? | - |
? | |
biuret + H2O | the enzyme acts uniquely on biuret physiologically | Rhizobium leguminosarum bv. viciae | allophanic acid + NH3 | - |
ir | |
butanediamide + H2O | low specific activity | Rhizobium leguminosarum bv. viciae | ? | - |
? | |
carbamoylcarbamate + H2O | low specific activity | Rhizobium leguminosarum bv. viciae | ? | - |
? | |
dicarbonimidic diamide + H2O | - |
Rhizobium leguminosarum bv. viciae | ? | - |
? | |
dicarbonimidic diamide + H2O | low specific activity | Rhizobium leguminosarum bv. viciae | ? | - |
? | |
dicarbonodithioimidic diamide + H2O | low specific activity | Rhizobium leguminosarum bv. viciae | ? | - |
? | |
ethanediamide + H2O | low specific activity | Rhizobium leguminosarum bv. viciae | ? | - |
? | |
ethanethioamide + H2O | low specific activity | Rhizobium leguminosarum bv. viciae | ? | - |
? | |
ethyl acetate + H2O | low specific activity | Rhizobium leguminosarum bv. viciae | ? | - |
? | |
ethyl carbamoylcarbamate + H2O | low specific activity | Rhizobium leguminosarum bv. viciae | ? | - |
? | |
formamide + H2O | low specific activity | Rhizobium leguminosarum bv. viciae | ? | - |
? | |
hexanediamide + H2O | low specific activity | Rhizobium leguminosarum bv. viciae | ? | - |
? | |
hydrazinecarbothioamide + H2O | low specific activity | Rhizobium leguminosarum bv. viciae | ? | - |
? | |
hydrazinecarboxamide + H2O | low specific activity | Rhizobium leguminosarum bv. viciae | ? | - |
? | |
N-carbamothioylacetamide + H2O | low specific activity | Rhizobium leguminosarum bv. viciae | ? | - |
? | |
N-carbamoylacetamide + H2O | - |
Rhizobium leguminosarum bv. viciae | ? | - |
? | |
N-carbamoylacetamide + H2O | low specific activity | Rhizobium leguminosarum bv. viciae | ? | - |
? | |
N-hydroxydicarbonimidic diamide + H2O | low specific activity | Rhizobium leguminosarum bv. viciae | ? | - |
? | |
N-nitrodicarbonimidic diamide + H2O | low specific activity | Rhizobium leguminosarum bv. viciae | ? | - |
? | |
prop-2-enamide + H2O | low specific activity | Rhizobium leguminosarum bv. viciae | ? | - |
? | |
propanediamide + H2O | low specific activity | Rhizobium leguminosarum bv. viciae | ? | - |
? | |
sulfuric diamide + H2O | low specific activity | Rhizobium leguminosarum bv. viciae | ? | - |
? | |
thiourea + H2O | low specific activity | Rhizobium leguminosarum bv. viciae | ? | - |
? | |
urea + H2O | low specific activity | Rhizobium leguminosarum bv. viciae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 27121, calculated from amino acid sequence | Rhizobium leguminosarum bv. viciae |
Synonyms | Comment | Organism |
---|---|---|
biuret hydrolase | - |
Rhizobium leguminosarum bv. viciae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4 | - |
Biuret | in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C | Rhizobium leguminosarum bv. viciae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Rhizobium leguminosarum bv. viciae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 11 | about 50% activity at pH 4.5 and pH 11.0 | Rhizobium leguminosarum bv. viciae |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Rhizobium leguminosarum bv. viciae | calculated from amino acid sequence | - |
5.7 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
170 | - |
Biuret | in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C | Rhizobium leguminosarum bv. viciae |