Literature summary for 3.5.1.83 extracted from

Moriguchi, M.; Sakai, K.; Katsuno, Y.; Maki, T.; Wakayama, M.
Purification and characterization of novel N-acyl-D-aspartate amidohydrolase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6 (1993), Biosci. Biotechnol. Biochem., 57, 1145-1148.

Search for more literature for 3.5.1.83

Inhibitors

Inhibitors	Comment	Organism	Structure
CaCl2	-	Achromobacter xylosoxidans
CoCl2	-	Achromobacter xylosoxidans
Cu2+	-	Achromobacter xylosoxidans
dithiothreitol	-	Achromobacter xylosoxidans
FeCl2	-	Achromobacter xylosoxidans
Hg2+	-	Achromobacter xylosoxidans
iodoacetic acid	-	Achromobacter xylosoxidans
MnCl2	-	Achromobacter xylosoxidans
NEM	-	Achromobacter xylosoxidans
Ni2+	-	Achromobacter xylosoxidans
PCMB	-	Achromobacter xylosoxidans
ZnCl2	-	Achromobacter xylosoxidans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.033	-	N-Butanoyl-D-Asp	-	Achromobacter xylosoxidans
0.194	-	N-Propanoyl-D-Asp	-	Achromobacter xylosoxidans
1.11	-	N-Glycyl-D-Asp	-	Achromobacter xylosoxidans
2.52	-	N-Acetyl-D-Asp	-	Achromobacter xylosoxidans
12.5	-	N-Formyl-D-Asp	-	Achromobacter xylosoxidans

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
54000	-	1 * 54000, SDS-PAGE	Achromobacter xylosoxidans
56000	-	gel filtration	Achromobacter xylosoxidans

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Achromobacter xylosoxidans	inducible by N-acetyl-D-Asp	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Achromobacter xylosoxidans	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Achromobacter xylosoxidans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
175	-	-	Achromobacter xylosoxidans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Gly-D-Asp + H2O	-	Achromobacter xylosoxidans	Glycine + Asp	-	?
additional information	no activity with N-carbobenzoyl-D-Asp, N-acetyl-L-Asp, N-acetyl-D-Glu	Achromobacter xylosoxidans	?	-	?
additional information	inducible by N-acetyl-D-Asp	Achromobacter xylosoxidans	?	-	?
N-Acetyl-D-Asp + H2O	-	Achromobacter xylosoxidans	acetate + D-Asp	-	?
N-Butanoyl-D-Asp + H2O	-	Achromobacter xylosoxidans	Butanoate + D-Asp	-	?
N-Formyl-D-Asp + H2O	-	Achromobacter xylosoxidans	Formate + D-Asp	-	?
N-glycyl-D-Asp + H2O	-	Achromobacter xylosoxidans	Gly + D-Asp	-	?
N-Propanoyl-D-Asp + H2O	-	Achromobacter xylosoxidans	Propanoate + D-Asp	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 54000, SDS-PAGE	Achromobacter xylosoxidans

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	-	Achromobacter xylosoxidans

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
45	-	10 min, stable up to	Achromobacter xylosoxidans
50	-	10 min, 40% loss of activity	Achromobacter xylosoxidans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	8	-	Achromobacter xylosoxidans

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
8	-	stable	Achromobacter xylosoxidans

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Release 2023.1 (January 2023)